IKKi/IKKe antibody (AA 701-716)

Catalog No. ABIN213741

The Rabbit Polyclonal anti-IKKi/IKKe antibody (ABIN213741) specifically detects IKKi/IKKe in WB, IHC and IHC (p). The antibody is reactive with Human samples.

Quick Overview for IKKi/IKKe antibody (AA 701-716) (ABIN213741)

Target: IKKi/IKKe (IKBKE) (Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase epsilon (IKBKE))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IKKi/IKKe antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-IKKi/IKKe Antibody

Binding Specificity: AA 701-716

Specificity: It has no cross response to IKKa, IKKb, or IKKy.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla (100%) Gibbon, Monkey (94%) Elephant (88%) Marmoset, Pig (81%).

Purification: Immunoaffinity purified

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (10 μg/mL), WB (0.5 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Not recommended for: IHC-P

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles. Store undiluted.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for 3 months and -20°C, stable for up to 1 year. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for IKKi/IKKe

Target: IKKi/IKKe (IKBKE) (Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase epsilon (IKBKE))

Alternative Name: IKBKE / IKKI / IKKE

Background: Name/Gene ID: IKBKE
Subfamily: IKAPPAB
Family: Protein Kinase

Synonyms: IKBKE, Ikb kinase epsilon, IKK-E, IKK-epsilon, Inducible I kappa-B kinase, KIAA0151, IKK-i, IKK-related kinase epsilon, I-kappa-B kinase epsilon, IKKE, IKKepsilon, IKKI, Inducible IkappaB kinase

Gene ID: 9641

UniProt: Q14164

Pathways: TLR Signaling, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades