CHEK2 antibody

Catalog No. ABIN213892

This Mouse Monoclonal antibody specifically detects CHEK2 in WB, IHC and IHC (p). It exhibits reactivity toward Human.

Quick Overview for CHEK2 antibody (ABIN213892)

Target: CHEK2 (Checkpoint Kinase 2 (CHEK2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CHEK2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 73C175-1-1

Product Details anti-CHEK2 Antibody

Specificity: A synthetic peptide obtained from human Chk2 protein sequence was used as immunogen.

Purification: Protein G purified

Immunogen: A synthetic peptide obtained from human Chk2 protein sequence.

Type of Immunogen: Synthetic peptide

Isotype: IgG1

Application Details

Application Notes: Approved: IHC, IHC-P (10 μg/mL), WB (2 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Short term: 4°C
Long term: -70°C.

Target Details for CHEK2

Target: CHEK2 (Checkpoint Kinase 2 (CHEK2))

Alternative Name: CHEK2 / CHK2

Background: Name/Gene ID: CHEK2
Subfamily: CDS1
Family: Protein Kinase

Synonyms: CHEK2, BA444G7, Cds1 homolog, Checkpoint kinase 2, Checkpoint-like protein CHK2, CHK2 checkpoint homolog, CHK2, HCds1, LFS2, RAD53, Rad53 homolog, HCHK2, HuCds1, PP1425

Gene ID: 11200

UniProt: O96017

Pathways: p53 Signaling, Apoptosis, Cell Division Cycle