CRADD antibody (AA 99-117)

Catalog No. ABIN213922

This Rabbit Polyclonal antibody specifically detects CRADD in WB, IHC, ICC and IHC (p). It exhibits reactivity toward Human.

Quick Overview for CRADD antibody (AA 99-117) (ABIN213922)

Target: CRADD (CASP2 and RIPK1 Domain Containing Adaptor with Death Domain (CRADD))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CRADD antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-CRADD Antibody

Binding Specificity: AA 99-117

Purification: Immunoaffinity purified

Isotype: IgG

Application Details

Application Notes: Approved: ICC, IHC, IHC-P (5 μg/mL), WB (1:500 - 1:1000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles. Store undiluted.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for 3 months and -20°C, stable for up to 1 year. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for CRADD

Target: CRADD (CASP2 and RIPK1 Domain Containing Adaptor with Death Domain (CRADD))

Alternative Name: CRADD / RAIDD

Background: Name/Gene ID: CRADD
Family: Apoptosis

Synonyms: CRADD, Death adaptor molecule RAIDD, RAIDD, MRT34

Gene ID: 8738

UniProt: P78560

Pathways: Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity