HIF1AN antibody (N-Term)

Catalog No. ABIN6736726

The Rabbit Polyclonal anti-HIF1AN antibody is suitable to detect HIF1AN in samples from Human, Rabbit and Horse. It has been validated for WB, IHC and IHC (p).

Quick Overview for HIF1AN antibody (N-Term) (ABIN6736726)

Target: HIF1AN (Hypoxia Inducible Factor 1, alpha Subunit Inhibitor (HIF1AN))

Reactivity: Human, Rabbit, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HIF1AN antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-HIF1AN Antibody

Binding Specificity: N-Term

Specificity: Human HIF1AN

Predicted Reactivity: Percent identity by BLAST analysis: Human (92%) Rabbit, Horse (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from N-Terminus of human HIF1AN (Q9NWT6, NP_060372). Percent identity by BLAST analysis: Human (100%), Gorilla, Monkey (92%), Gibbon, Marmoset, Elephant, Rabbit, Horse (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for HIF1AN

Target: HIF1AN (Hypoxia Inducible Factor 1, alpha Subunit Inhibitor (HIF1AN))

Alternative Name: HIF1AN

Background: Name/Gene ID: HIF1AN

Synonyms: HIF1AN, Factor inhibiting HIF-1, FIH1, Factor inhibiting HIF1, FIH-1

Gene ID: 55662

NCBI Accession: NP_060372

UniProt: Q9NWT6

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development