POLR2B antibody (AA 805-854)

Catalog No. ABIN6736747

The Rabbit Polyclonal anti-POLR2B antibody is suitable to detect POLR2B in samples from Human, Mouse, Rat, Dog, Cow, Guinea Pig, Horse, Rabbit, Pig, Xenopus laevis and Monkey. It has been validated for WB, IHC and IHC (p).

Quick Overview for POLR2B antibody (AA 805-854) (ABIN6736747)

Target: POLR2B (Polymerase (RNA) II (DNA Directed) Polypeptide B, 140kDa (POLR2B))

Reactivity: Human, Mouse, Rat, Dog, Cow, Guinea Pig, Horse, Rabbit, Pig, Xenopus laevis, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLR2B antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-POLR2B Antibody

Binding Specificity: AA 805-854

Specificity: Human POLR2B / RPB2

Predicted Reactivity: Percent identity by BLAST analysis: Human, Rat, Dog, Bovine, Xenopus (100%) Chicken, Zebrafish (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa805-854 of human POLR2B (P30876, NP_000929). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Dog, Bovine, Rabbit, Horse, Pig, Opossum, Guinea pig, Platypus, Xenopus (100%), Zebra finch, Chicken, Salmon, Stickleback, Zebrafish (92%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: After adding water, will consist of PBS buffer with 2 % sucrose

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for POLR2B

Target: POLR2B (Polymerase (RNA) II (DNA Directed) Polypeptide B, 140kDa (POLR2B))

Alternative Name: POLR2B / RPB2

Background: Name/Gene ID: POLR2B

Synonyms: POLR2B, HsRPB2, POL2RB, RNA polymerase II subunit B2, RNA polymerase II subunit 2, RPB2, HRPB140

Gene ID: 5431

NCBI Accession: NP_000929

UniProt: P30876

Pathways: Regulatory RNA Pathways, DNA Damage Repair