FYN antibody (AA 7-176)

Catalog No. ABIN214218

The Mouse Monoclonal anti-FYN antibody (Clone FYN-59) (ABIN214218) specifically detects FYN in WB, IHC, IP and IHC (p). The antibody is reactive with Human and Mouse samples.

Quick Overview for FYN antibody (AA 7-176) (ABIN214218)

Target: FYN (FYN Oncogene Related To SRC, FGR, YES (FYN))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This FYN antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: FYN-59

Product Details anti-FYN Antibody

Binding Specificity: AA 7-176

Specificity: Partial recombinant human Fyn (aa 7-176) expressed in E coli

Purification: Affinity purified

Immunogen: Recombinant human FYN.

Type of Immunogen: Recombinant protein

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (15 μg/mL), IP, WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 15 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Phosphate-buffered solution,  pH 7.2, 0.09 % sodium azide at 0.5 mg/mL.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freezing

Storage: 4 °C

Storage Comment: 4°C, avoid freezing.

Target Details for FYN

Target: FYN (FYN Oncogene Related To SRC, FGR, YES (FYN))

Alternative Name: SYN / FYN

Background: Name/Gene ID: FYN
Subfamily: Src
Family: Protein Kinase

Synonyms: FYN, C-syn protooncogene, Fyn kinase, Fyn tyrosine kinase, p59-FYN, SYN, Src-like kinase, Src/yes-related novel, Tyrosine kinase fyn, Tyrosine kinase p59fyn(T), Tyrosine-protein kinase Fyn, Proto-oncogene c-Fyn, Proto-oncogene Syn

Gene ID: 2534

UniProt: P06241

Pathways: JAK-STAT Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Feeding Behaviour, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Activated T Cell Proliferation, Thromboxane A2 Receptor Signaling