PPP1R13L antibody (AA 780-797)

Catalog No. ABIN214371

Product Details anti-PPP1R13L Antibody

Target: PPP1R13L (Protein Phosphatase 1, Regulatory Subunit 13 Like (PPP1R13L))

Binding Specificity: AA 780-797

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PPP1R13L antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Amino acids 780-797 of human iASPP (isoform 1) protein.

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from human PPP1R13L / IASPP.

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: ELISA (1:40000 - 1:160000), IF, IHC, IHC-P (2.5 μg/mL), WB (1:1000 - 1:5000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 0.01 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid cycles of freezing and thawing.

Storage: -20 °C

Storage Comment: Store vial at -20°C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.

Target Details for PPP1R13L

Target: PPP1R13L (Protein Phosphatase 1, Regulatory Subunit 13 Like (PPP1R13L))

Alternative Name: PPP1R13L / RAI / IASPP (PPP1R13L Products)

Synonyms: AV260376 antibody, IASPP antibody, Nkip1 antibody, wa3 antibody, zgc:113058 antibody, NKIP1 antibody, RAI antibody, RAI4 antibody, protein phosphatase 1, regulatory (inhibitor) subunit 13 like antibody, protein phosphatase 1, regulatory subunit 13 like antibody, protein phosphatase 1 regulatory subunit 13 like antibody, protein phosphatase 1 regulatory subunit 13 like L homeolog antibody, Ppp1r13l antibody, ppp1r13l antibody, PPP1R13L antibody, ppp1r13l.L antibody

Background: Name/Gene ID: PPP1R13L

Synonyms: PPP1R13L, IASPP, Inhibitor of ASPP protein, NFkB-interacting protein 1, NKIP1, RAI4, RAI, Retinoic acid induced 4, NFkB interacting protein 1, PPP1R13BL, Protein iASPP, RelA-associated inhibitor, PPP1R13B-like protein

Gene ID: 10848

UniProt: Q8WUF5