CHRNB2 antibody (AA 21-70)

Catalog No. ABIN6736368

The Rabbit Polyclonal anti-CHRNB2 antibody is suitable to detect CHRNB2 in samples from Human, Rat, Mouse, Dog, Cow, Pig, Guinea Pig, Zebrafish (Danio rerio), Bat, Monkey, Xenopus laevis and Horse. It has been validated for WB, IHC and IHC (p).

Quick Overview for CHRNB2 antibody (AA 21-70) (ABIN6736368)

Target: CHRNB2 (Cholinergic Receptor, Nicotinic, beta 2 (Neuronal) (CHRNB2))

Reactivity: Human, Rat, Mouse, Dog, Cow, Pig, Guinea Pig, Zebrafish (Danio rerio), Bat, Monkey, Xenopus laevis, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CHRNB2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-CHRNB2 Antibody

Binding Specificity: AA 21-70

Specificity: Human CHRNB2

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Dog, Bovine, Pig (100%) Xenopus (92%) Zebrafish (83%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa21-70 of human CHRNB2 (P17787, NP_000739). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Monkey, Galago, Marmoset, Mouse, Rat, Ferret, Elephant, Panda, Dog, Bovine, Bat, Horse, Pig, Guinea pig, Xenopus (100%), Opossum, Sea squirt (92%), Zebra finch, Chicken (85%), Turkey (84%), Pufferfish, Zebrafish (83%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (10 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for CHRNB2

Target: CHRNB2 (Cholinergic Receptor, Nicotinic, beta 2 (Neuronal) (CHRNB2))

Alternative Name: CHRNB2

Background: Name/Gene ID: CHRNB2
Subfamily: Nicotinic acetylcholine receptor
Family: Ion Channel

Synonyms: CHRNB2, AChR-beta1, Acrb2, EFNL3, NAChR beta 2, NAChRB2

Gene ID: 1141

NCBI Accession: NP_000739

UniProt: P17787

Pathways: Sensory Perception of Sound, Feeding Behaviour