HSP90AA1 antibody (AA 604-697)

Catalog No. ABIN215213

The Mouse Monoclonal anti-HSP90AA1 antibody is suitable to detect HSP90AA1 in samples from Human, Mouse, Rat, Monkey, Rabbit, Cow, Chicken, Guinea Pig, Pig, Sheep, Dog, Hamster, Water Mold and C. elegans. It has been validated for WB and ICC.

Quick Overview for HSP90AA1 antibody (AA 604-697) (ABIN215213)

Target: HSP90AA1 (Heat Shock Protein 90kDa alpha (Cytosolic), Class A Member 1 (HSP90AA1))

Reactivity: Human, Mouse, Rat, Monkey, Rabbit, Cow, Chicken, Guinea Pig, Pig, Sheep, Dog, Hamster, Water Mold, C. elegans

Host: Mouse

Clonality: Monoclonal

Conjugate: This HSP90AA1 antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC)

Product Details anti-HSP90AA1 Antibody

Binding Specificity: AA 604-697

Specificity: Identifies purified bovine and human (alpha and beta) Hsp90 and detects a 90kD protein, corresponding to the apparent molecular mass of Hsp90 on SDS-PAGE immunoblots, in samples from human, mouse, rat, beluga, bovine, canine, C. elegans, chicken, fish (brook trout, carp, chinook salmon, chum salmon, medaka, rainbow trout), guinea pig, hamster, lobster, monkey, mussel, porcine, rabbit, scallop, sheep and water mold. Reacts with rodent glucocorticoid receptor, 95kD. Also reacts with proteins called proliferation potential proteins (P2PS), ~30-40kD. These proteins are associated with the 30-40S substructures of nuclear hnRNP complexes and they share an epitope in common with Hsp90. The epitope has been mapped to amino acids 604-697 of the human Hsp90 sequence. Detects both Hsp90 alpha and beta.

Purification: Protein G purified

Immunogen: Achlya ambisexualis (water mold) Hsp90.

Isotype: IgG1

Application Details

Application Notes: Approved: ICC, WB

Usage: Suitable for use in Western Blot, Immunoprecipitation, Immunocytochemistry, Immunohistochemistry and FACS. Western Blot (Colorimetric): 1 μg/mL. Immunoprecipitation: 4 μg/mL. Does not appear to react well with Hsp90 that is bound to steroid receptors or to pp60v-src of Rouse sarcoma virus. Positive control: Hsp90 (Human) Protein and HeLa (Heat Shocked) Cell Lysate.

Comment:

Target Species of Antibody: Achlya ambisexualis

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 10 mM PBS,  pH 7.2, 0.1 mM PMSF, 50 % glycerol.

Preservative: PMSF

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for HSP90AA1

Target: HSP90AA1 (Heat Shock Protein 90kDa alpha (Cytosolic), Class A Member 1 (HSP90AA1))

Alternative Name: HSP90AA1 / Hsp90 Alpha A1

Background: Name/Gene ID: HSP90AA1

Synonyms: HSP90AA1, EL52, HSP86, Hsp89, HSP89A, HSPC1, Heat shock 86 kDa, Hsp90, HSP90A, HSP90N, HSPCA, HSPCAL4, HSPN, LAP2, HSP 86, HSPCAL1

Gene ID: 3320

UniProt: P07900

Pathways: M Phase, Regulation of Cell Size, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals