BID antibody

Catalog No. ABIN2964738

This Rabbit Polyclonal antibody specifically detects BID in WB. It exhibits reactivity toward Mouse.

Quick Overview for BID antibody (ABIN2964738)

Target: BID (BH3 Interacting Domain Death Agonist (BID))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BID antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-BID Antibody

Specificity: Recognizes the 15kD fragment of mouse BH3 interacting domain death agonist (BID), a product of caspase-8 cleavage during apoptosis. It does not recognize the full length mouse BID or cleaved human BID. Species sequence homology: human (75 % ), rat (100 %), chicken (62 %).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide corresponding to cleavage site (59/60) of mouse BID.

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (1:1000)

Usage: Suitable for use in Western Blot. Western Blot: 1:1000. At 0.5 μg/mL, the dilution provides 100 mL working solution, which at 10 ml/blot allows 10 blots to be performed. Positive control: TNFa treated mouse L929 cells.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Dulbecco's PBS (without Mg2+, Ca2+),  pH 7.2, 1 % BSA, 0.05 % sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for BID

Target: BID (BH3 Interacting Domain Death Agonist (BID))

Alternative Name: BID

Background: Name/Gene ID: BID
Family: Apoptosis

Synonyms: BID, Apoptic death agonist, BID isoform L(2), BID isoform Si6, Bh3, FP497, p22 BID, BID isoform ES(1b), Desmocollin type 4, Human BID coding sequence

Gene ID: 637

UniProt: P55957

Pathways: Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity