FK506 Binding Protein 8, 38kDa (FKBP8) antibody

Antigen: FK506 Binding Protein 8, 38kDa (FKBP8)

Synonyms: 38kDa, Fkbp38, FKBP38, FKBPr38, FKBP-8, MGC125185, FKBP8, MGC77672, zgc:77672, fkbp8

Clonality: Polyclonal

Host: Rabbit

Reactivity: Mouse (Murine), Human, Rat (Rattus)

Application: ELISA, Western Blotting (WB), Immunoprecipitation (IP)

Catalog no.: ABIN233735

Additional Information

Alternative name: FKBP8

Gene ID: NP_036313

Swiss-Prot: Q86YK6

Immunogen: This protein A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human FKBP8 protein.

Format: Liquid (sterile filtered)

Description: FKBP8 (also known as FK506-binding protein 38) is a member of the immunophilin family that has been implicated to play an important role in apoptosis through its involvement in the mechanism that targets Bcl-2 and Bcl-xL to the outer mitochondrial membrane (OMM). Suppression of endogenous FKBP8 by RNAi or transfection of a mutant FKBP8 missing the transmembrane domain necessary for mitochondrial insertion, resulted in the translocation of Bcl-2 and Bcl-xL from the OMM to the cytosol. It has also been suggested that FKBP8 may play a role in the cross-talk between the Bcl-2 and the Calcineurin/NF-AT apoptosis signaling pathways and in cell size regulation through interactions with tuberous sclerosis genes.

Specificity: This product was protein A purified from monospecific antiserum by immunoaffinity chromatography using protein A coupled to agarose beads. This antibody is specific for human FKBP8 protein. A BLAST analysis was used to suggest partial cross-reactivity with FKBP8 from mouse and rat sources based on ~93% homology with the immunizing sequence. Cross-reactivity with FKBP8 from other sources has not been determined.

Application Details

Application Notes: Recommended Dilutions: ELISA 1:25,000 WESTERN BLOT 1:500 - 1:2,000 IMMUNOHISTOCHEMISTRY User Optimized OTHER APPLICATIONS User Optimized. NoteThis protein A purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 64 kDa in size corresponding to FKBP8 by western blotting in the appropriate cell lysate or extract.

Concentration: 2.3 mg/ml (by UV absorbance at 280 nm)

Buffer: Stabilizer: None. Preservative: 0.01% (w/v) Sodium Azide. Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2.

Storage: Store vial at -20° C or below prior to opening. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of opening.

Restrictions: For Research Use only

Images

Western blot using antibodies-online's protein A purified anti-FKBP8 antibody shows detection of exogenous FKBP8 in 50 ug of HEK293T whole cell lysate (lane 1). The results of peptide competition are shown in lane 2 where no specific staining is noted after the antibody is first incubated for 1h with the immunizing peptide in 5% BLOTTO prior to reaction with the membrane. The membrane was probed with the primary antibody at a 1:1,000 dilution in 5% BLOTTO at 4

anti-FK506 Binding Protein 8, 38kDa (FKBP8) antibody (Image 2)