CD57 antibody

Catalog No. ABIN234706

This Mouse Monoclonal antibody specifically detects CD57 in FACS. It exhibits reactivity toward Human.

Quick Overview for CD57 antibody (ABIN234706)

Target: CD57 (B3GAT1) (beta-1,3-Glucuronyltransferase 1 (Glucuronosyltransferase P) (B3GAT1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD57 antibody is un-conjugated

Application: Flow Cytometry (FACS)

Clone: VC1-1

Product Details anti-CD57 Antibody

Specificity: Recognizes a (Mr 110 kDa) carbohydrate molecule on myeloid cells. This surface antigen is associated with myelin-associated glycoprotein (MAG).(1) This antibody blocks binding of HNK-1 (Leu. 7) antibody.(3) The CD57 antigen is present on 15-20% of normal peripheral blood mononuclear cells. It is expressed on a subset of natural killer cells (60%) and on a subset of T-lymphocytes. This carbohydrate is also present on N-CAM in the nervous system.

Characteristics: MAb to CD57, Monoclonal antibody to Human CD57/HNK-1 (Natural Killer cells)

Purification: Protein G Chromatography. Tissue culture

Immunogen: Cat unfixed cortical homogenate area 17

Isotype: IgM

Application Details

Application Notes: Suitable for use in flow cytometry, Western blotting and immunohistochemistry (formalin fixed paraffin sections). We recommend using 1 µg to stain 1.0 x 106 cells in flow cytometric applications. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 0.01 M PBS, pH 7.2 containing 1 % BSA, 0.01 % Sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid multiple freeze/thaw cycles.
Centrifuge product if not completely clear after standing at room temperature.
Prepare working dilution only prior to immediate use.

Storage: 4 °C/-20 °C

Storage Comment: Short term (up to one month) store at 2-8 °C. Long term, aliquot and store at -28 °C.

Expiry Date: 1 month

Target Details for CD57

Target: CD57 (B3GAT1) (beta-1,3-Glucuronyltransferase 1 (Glucuronosyltransferase P) (B3GAT1))

Pathways: Glycosaminoglycan Metabolic Process