Epidermal Growth Factor Receptor (EGFR) (prylated at Serine 1047) antibody

Antigen: Epidermal Growth Factor Receptor (EGFR)

Synonyms: ERBB, HER1, mENA, ERBB1, PIG61, Wa5, wa2, Erbb, Errp, wa-2, AI552599, 9030024J15Rik, ErbB-1, C-erb, D-EGFR, D-Egf, DEGFR, DER, DER flb, DER/EGFR, DER/faint little ball, DER/top, DER/torpedo, DmHD-33, EFG-R, EGF-R, EGFR, EK2-6, Egf, El, Elp, Elp-1, Elp-B1, Elp-B1RB1, HD-33, Torpedo/DER, Torpedo/Egfr, c-erbB, d-egf-r, dEGFR1, flb, l(2)05351, l(2)09261, l(2)57DEFa, l(2)57EFa, l(2)57Ea, pnt, top, top/DER, top/flb, DmelCG10079, CG10079, egfr, EGFR12, EGFR15, egfr1, Erbb2

Epitope: prylated at Serine 1047

Clonality: Monoclonal (1H9)

Host: Mouse

Reactivity: Human

Application: ELISA, Immunofluorescence (IF), Western Blotting (WB)

Catalog no.: ABIN235074

Additional Information

Alternative name: Human Epidermal Growth Factor Receptor (EGFR), phosphorylated at Serine 1047

Immunogen: Synthetic phosphopeptide corresponding to amino acid residues surrounding Serine 1047 Format: Purified, Lyophilized. Reconstitute with 1ml water for 15 minutes at room temperature.

Format: Purified, Lyophilized

Isotype: IgG1, kappa chain  (Matching secondary antibodies)

Clone: 1H9

Description: Cell lysate of 2 x 106 vanadate treated HepG2 cells.

Specificity: The EGF Receptor (EFR) is activated upon stimulation with growth factors of the EGF family and autophosphorylates at multiple tyrosine residues. Serine phosphorylation of the carboxy terminal domain at Serine 1047 leads to ligand-dependent EGFR desensitization. However, if serine 1047 is phosphorylated, the neighboring tyrosine 1045 may not be phosphorylated due to sterical hindrance, thus inhibiting cbl-mediated receptor degradation. Clone 1H9 specifically recognizes EGFR phosphorylated at Serine 1047. Reacts with Human, mouse and dog.

Synonyms: ERBB, HER1, mENA, ERBB1, PIG61, Wa5, wa2, Erbb, Errp, wa-2, AI552599, 9030024J15Rik, ErbB-1, C-erb, D-EGFR, D-Egf, DEGFR, DER, DER flb, DER/EGFR, DER/faint little ball, DER/top, DER/torpedo, DmHD-33, EFG-R, EGF-R, EGFR, EK2-6, Egf, El, Elp, Elp-1, Elp-B1, Elp-B1RB1, HD-33, Torpedo/DER, Torpedo/Egfr, c-erbB, d-egf-r, dEGFR1, flb, l(2)05351, l(2)09261, l(2)57DEFa, l(2)57EFa, l(2)57Ea, pnt, top, top/DER, top/flb, DmelCG10079, CG10079, egfr, EGFR12, EGFR15, egfr1, Erbb2

Application Details

Application Notes: Immunoblotting: 0.5ug/ml for HRP/ECL detection. Recommended blocking buffer CPPT: 0.5% (w/v) casein, 1% (w/v) PEG 4000, 1% (w/v) Polyvinylpyrrolidone (PVP), 0.1% Tween 20, 10mM Tris/HCl, pH 7.4, 150mM Sodium Chloride. EGFR Transactivation Serum starved HepG2 cells were treated for 15 minutes as indicated. Whole cell lysates were separated by SDS-PAGE (20,000 cell per lane). The blot was probed with 0.5?g/ml K67190M for 1 hour at RT and developed by ECL (exp. time: 30 sec). Lane 1: control Lane 5: Sorbit Lane 2: PMA Lane 6: Anisomycin Lane 3: Forskolin Lane 7: Lonomycin Lane 4: LPA Lane 8: Taxol FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. Catalog K67190M Page 2 of 2 Immunocytochemistry: 0.1-1ug/ml ELISA: 0.1ug/ml Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded. Storage: Store lyophilized product at ?20?C. After reconstitution, aliquot and store at ?80?C. Thawed aliquots may be stored at 2-8?C for up to 3 months. Warning:

Concentration: 100ug/ml (prior to lyophilization)

Purification: Thiophilic adsorption and size exclusion chromatography. Source: Cell culture

Buffer: Lyophilized from PBS, PEG, and Sucrose. Preservative: 0.09% Sodium azide

Storage: Store lyophilized product at -20°C. After reconstitution, aliquot and store at -80°C. Thawed aliquots may be stored at 2-8°C for up to 3 months. Warning:Reconstitute with 1ml water for 15 minutes at room temperature. Purification:

Restrictions: For Research Use only

Images

EGFR Transactivation: Serum starved HepG2 cells were treated for 15 minutes as indicated. Whole cell lysates were separated by SDS-PAGE (20,000 cell per lane). The blot was probed with 0.5?g/ml ABIN235074 for 1 hour at RT and developed by ECL (exp. time: 30 sec). Lane 1: control Lane 5: Sorbit Lane 2: PMA Lane 6: Anisomycin Lane 3: ForskolinLane 7: Lonomycin Lane 4: LPALane 8: Taxol