Epidermal Growth Factor Receptor (EGFR) (prylated At Tyrosine 1173) antibody

Antigen: Epidermal Growth Factor Receptor (EGFR)

Synonyms: ERBB, HER1, mENA, ERBB1, PIG61, Wa5, wa2, Erbb, Errp, wa-2, AI552599, 9030024J15Rik, ErbB-1, C-erb, D-EGFR, D-Egf, DEGFR, DER, DER flb, DER/EGFR, DER/faint little ball, DER/top, DER/torpedo, DmHD-33, EFG-R, EGF-R, EGFR, EK2-6, Egf, El, Elp, Elp-1, Elp-B1, Elp-B1RB1, HD-33, Torpedo/DER, Torpedo/Egfr, c-erbB, d-egf-r, dEGFR1, flb, l(2)05351, l(2)09261, l(2)57DEFa, l(2)57EFa, l(2)57Ea, pnt, top, top/DER, top/flb, DmelCG10079, CG10079, egfr, EGFR12, EGFR15, egfr1, Erbb2

Epitope: prylated At Tyrosine 1173

Clonality: Monoclonal (9H2)

Host: Mouse

Reactivity: Human

Application: ELISA, Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)

Catalog no.: ABIN235075

Additional Information

Alternative name: Human Epidermal Growth Factor Receptor (EGFR), phosphorylated at Tyrosine 1173

Immunogen: Phosphopeptide conjugated to KLH Format: Purified, Lyophilized Reconstitute with 1ml water for 15 minutes at room temperature.

Format: Purified, Lyophilized

Isotype: IgG1  (Matching secondary antibodies)

Clone: 9H2

Description: Cell lysate of pervanadate-treated HepG2 cells Cell culture

Specificity: Binding of EGF to the extracellular domain of the EGF-receptor results in receptor dimerization and autophosphorylation on tyrosine residues, Y1173 being the major autophosphorylation site. Clone 9H2 specifically interacts with the 1170 ? NAEpYLRV motif corresponding to the major autophosphorylation site of human EGFR. The antibody does not interact with the non-phosphorylated EGFR nor with unrelated Tyrosine-phosphorylated proteins. This phosphorylation site?specific monoclonal antibody allows the detection and quantification of activated EGFR in crude cell extracts in Immunoblot, ELISA and Biosensor assay formats without the use of P32. Reacts with Human, mouse, and dog.

Synonyms: ERBB, HER1, mENA, ERBB1, PIG61, Wa5, wa2, Erbb, Errp, wa-2, AI552599, 9030024J15Rik, ErbB-1, C-erb, D-EGFR, D-Egf, DEGFR, DER, DER flb, DER/EGFR, DER/faint little ball, DER/top, DER/torpedo, DmHD-33, EFG-R, EGF-R, EGFR, EK2-6, Egf, El, Elp, Elp-1, Elp-B1, Elp-B1RB1, HD-33, Torpedo/DER, Torpedo/Egfr, c-erbB, d-egf-r, dEGFR1, flb, l(2)05351, l(2)09261, l(2)57DEFa, l(2)57EFa, l(2)57Ea, pnt, top, top/DER, top/flb, DmelCG10079, CG10079, egfr, EGFR12, EGFR15, egfr1, Erbb2

Application Details

Application Notes: Immunoblotting: 0.5 ug/ml for HRP/ECL detection. Recommended blocking buffer CPPT: 0.5% (w/v) casein, 1% (w/v) PEG 4000, 1% (w/v) Polyvinylpyrrolidone (PVP), 0.1% Tween 20, 10mM Tris/HCl, pH 7.4, 150mM Sodium Chloride. Phosphospecificity Whole cell extracts of control (co), EGF stimulated (EGF) or pervanadate treated (VH) A549 tumor cells were applied to SDS-PAGE (20,000 cells per lane) and transferred to a PVDF membrane. The blot was probed with 0.5?g/ml K67902M for 1 hour at RT and developed by ECL (exp. time: 30 sec). Immunoprecipitation: 1?10ug per 106 vanadate treated A431 cells. Immunocytochemistry: 1?10ug/ml ELISA: 0.05ug/ml Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded. Storage: Store lyophilized product at -20?C. After reconstitution, aliquot and store at -80?C. Thawed aliquots may be stored at 2?8?C for up to 3 months. Warning:

Concentration: 100ug/ml (prior to lyophilization)

Purification: Thiophilic adsorption and size exclusion chromatography. Source: Cell culture

Buffer: Lyophilized from PBS, PEG and Sucrose. Preservative: 0.09% Sodium azide

Storage: Store lyophilized product at -20°C. After reconstitution, aliquot and store at -80°C. Thawed aliquots may be stored at 2-8°C for up to 3 months. Warning:Reconstitute with 1ml water for 15 minutes at room temperature. Purification:

Restrictions: For Research Use only

Images

Phosphospecificity: Whole cell extracts of control (co), EGF stimulated (EGF) or pervanadate treated (VH) A549 tumor cells were applied to SDS-PAGE (20,000 cells per lane) and transferred to a PVDF membrane. The blot was probed with 0.5?g/ml ABIN235075 for 1 hour at RT and developed by ECL (exp. time: 30 sec).