LAT2 antibody (AA 91-243)

Catalog No. ABIN238343

The Rabbit Polyclonal anti-LAT2 antibody is suitable to detect LAT2 in samples from Human. It has been validated for WB and IHC (p).

Quick Overview for LAT2 antibody (AA 91-243) (ABIN238343)

Target: LAT2 (Linker For Activation of T Cells Family, Member 2 (LAT2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LAT2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-LAT2 Antibody

Binding Specificity: AA 91-243

Specificity: The polyclonal antibody recognizes the defined epitope (aa 91-243) of human Non-T cell Activation Linker (NTAL), also known as LAB (linker of activated B cells), a 30 kDa transmembrane adaptor protein present in membrane microdomains (rafts) of B cells, NK cells and myeloid cells.

Purification: Protein-A affinity chromatography.

Immunogen: Recombinant cytoplasmic domain (aa 91-243) of human NTAL.

Application Details

Application Notes: Suitable for Western blot under Non-Reducing conditions: 15 % separating SDS-PAGE gel. Recommended dilution: 1 μg/mL, incubation 60 min in RT, or overnight at 4 °C. Positive control: RAMOS human lymphoma cell line. THP-1 human monocytic leukemia cell line. Negative control: JURKAT human T cell leukemia cell line. Sample preparation: Resuspend approx.50 mil. cells in 1 mLcold Lysis buffer (1 %laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Proteaseinhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysatewith non-reducing SDS-PAGE sample buffer. Immunohistochemistry on paraffin sections: 10 μg/mL. Positive tissue: spleen.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4 with 15 mM sodium azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for LAT2

Target: LAT2 (Linker For Activation of T Cells Family, Member 2 (LAT2))

Alternative Name: LAT2 / WBSCR15

Background: NTAL (Non-T cell activation linker), also known as LAB (Linker for activation of B cells), is a 30 kDa double-palmitoylated transmembrane adaptor protein expressed by B cells, NK cells, mast cells and macrophages. It is a negative regulator of early stages of BCR-dependent B cell signaling and serves as a negative regulator also in mast cells. However, in mast cells, NTAL also contributes to some activation processes, partially overlapping with LAT function.Synonyms: HSPC046, LAB, LLinker for activation of B-cells, Linker for activation of T-cells family member 2, Membrane-associated adapter molecule, NTAL, Non-T-cell activation linker, WBS15, WBSCR5, Williams-Beuren syndrome chromosomal region 15 protein

Gene ID: 7462

NCBI Accession: NP_054865

UniProt: Q9GZY6

Pathways: Fc-epsilon Receptor Signaling Pathway, BCR Signaling