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CD4 Molecule (CD4) antibody

Antigen

CD4 Molecule (CD4)
Synonyms CD4, MGC165891, cd4, CD4mut, L3T4, Ly-4, p55, W3/25
Clonality Monoclonal (13B8-2)
Host
Alternatives

Mouse
Reactivity
Alternatives

Human
Application
Alternatives Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Functional Studies (Func), Western Blotting (WB)
Catalog no. ABIN238364
Quantity 0.2 mg  (0.2 mg/ml (prior to lyophilization))
Price 430.00 $   Plus shipping costs $35.00
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Availability Ships within 7 to 10 Business Days

Additional Information
Application Details Alternatives Secondary Antibodies back to top

Characteristics Synonyms: T4/Leu-3, T-cell surface glycoprotein CD4, T-cell surface antigen T4/Leu-3
Alternative name CD4
Swiss-Prot P01730
Immunogen Human thymocytes
Cross-Reactivity Human
Isotype IgG1  (Matching secondary antibodies)
Clone 13B8-2
Description CD4 is a single chain transmembraneous glycoprotein (59 kDa) which belongs to theimmunoglobulin superfamily. CD4 is present on a subset of T lymphocytes(helper/inducer T cells) and is also expressed at a lower level on monocytes, tissuemacrophages and granulocytes. The antigen is involved in binding to MHC class IImolecules. The intracellular domain of the antigen is associated with p56lck proteintyrosine kinase.
Specificity The CD4 antigen is a monomeric transmembrane glycoprotein with a molecular weight (Mr)of 60 kDa. CD4 molecule is expressed on a specific subset of peripheral blood T lymphocytes named''helper/inducer''. The CD4 antige is present on approximately 45% of peripheral bloodlymphocytes (T4 lymphocytes), 80% of thymocytes and with a lower density of 100% ofmonocytes and on some neutrophils. Add. Information: CD4 acts as an accessory molecule to the T cell receptor (TcR) complex during T-cellactivation restricted to the Class II major Hitocompatibility Complex (HHC). The CD4 antigen is also known to be one of the human Immunodeficiency Virus (HIV)receptors. The T lymphocyte subset that expresses CD4 is involved in T-T, T-B, and B-MacrophageCellular Interactions.

Application Details
Alternatives Secondary Antibodies back to top

Application Notes Flow cytometry: Studies of CD4 expressing cells. Fluorescence microscopy or Flow cytometry: 2 g/5x10e5 cells/test. Immunohistochemistry: Working dilution 1/25-1/50. Only suitable on cryostat sections or cell smears. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Concentration 0.2 mg/ml (prior to lyophilization)
Purification Azide Free
Buffer PBS containing 1 mg/ml BSA as stabilizer without preservatives.Reconstitution: Reconstitute with 1 ml distilled water.
Storage Store lyophilized (preferably in a desiccator) at 2-8°C and reconstituted (in aliquots) at-20°C. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Research Area Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells
Restrictions For Research Use only

Alternatives
Application Details Secondary Antibodies back to top

Alternatives for antigen "CD4 Molecule (CD4)", type "Antibodies"
Hosts Mouse (444), Rat (208), Rabbit (68)
Reactivities Human (320), Mouse (Murine) (239), Rat (Rattus) (106), Dog (Canine) (44), Chicken (34), Cow (Bovine) (34), Sheep (Ovine) (24), Pig (Porcine) (22), Cat (Feline) (20), Rabbit (20), Bird (Avian) (8), Guinea Pig (8), Horse (Equine) (4), Duck (3), Monkey (3)
Applications Flow Cytometry (FACS) (601), Immunohistochemistry (Frozen Sections) (IHC (fro)) (148), Immunoprecipitation (IP) (93), Western Blotting (WB) (82), Immunofluorescence (IF) (53), ELISA (50), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (49), Functional Studies (Func) (34), Immunohistochemistry (IHC) (29), Immunocytochemistry (ICC) (16), Blocking Antibody (Inhibition) (11), Cytotoxicity Test (CyTox) (11), Enzyme Immunoassay (EIA) (8), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (4), Immunoelectron Microscopy (IEM) (2), Blocking Antibody (Inhibition) (1), ELISA (Detection) (1), Immunohistochemistry (Fixed) (IHC (fx)) (1), Neutralization (Neut) (1)
Conjugates FITC (116), PE (77), Biotin (71), APC (40), RPE (22), Cy5 (9), Cy5,PE (9), Cy5.5 (6), Cy7,PE (6), Alexa Fluor 488 (5), PE,Cy7 (5), HRP (4), PE,Cy5.5 (4), Pacific Blue (4), APC,Cy7 (3), PE,Cy5 (3), PerCP (3), SPRD (3), Alexa Flour 488 (2), Alexa Fluor 350 (2), Alexa Fluor 555 (2), Alexa Fluor 647 (2), Alexa Fluor 700 (2), Cy3 (2), Cy7 (2), Gold (2), PE,Cy3 (2), RBITC (2), DY647 (1), Dyomics 647 (1), PE,DY590 (1), PE,DY647 (1), PE,Texas Red (TR) (1), PE-Dyomics 590 (1), PE-Dyomics 647 (1), PerCP,Cy5.5 (1), RPE,Cy5 (1), RPE,Cy7 (1)
Epitopes domain 1 (10), domain 2 (5), non-polymorphic (5)