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Antibodies
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anti-Acrosomal Vesicle Protein 1 (ACRV1) antibody (FITC)
Acrosomal Vesicle Protein 1 (ACRV1) antibody (FITC)
| Antigen |
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| Synonyms |
SP-10, SPACA2, D11S4365, Sp10, ACRV1, Msa63 |
| Clonality |
Monoclonal (Hs-14) |
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Host
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Reactivity
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Conjugate
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FITC
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Application
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| Catalog no. |
ABIN238419 |
| Quantity |
50 Tests |
| Price |
390.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 7 to 10 Business Days |
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Characteristics
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Synonyms: Acrosomal protein SP-10, SP10, SPACA2, Acrosomal vesicle protein 1, Intra-acrosomalSperm Protein
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Alternative name
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ACRV1
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Gene ID
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56
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Swiss-Prot
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P26436
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Immunogen
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Freshly ejaculated Human sperms were washed in PBS and extracted in 3% Acetic Acid,10% Glycerol, 30mM benzaminidine. The acid extract was dialyzed against 0. 2% AceticAcid and subsequently used for immunization.
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Cross-Reactivity
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Human, Mouse (Murine)
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Isotype
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IgM
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Clone
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Hs-14
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Description
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One of the most frequent causes of man infertility is defective sperm acrosome. Thisdamage can be detected using antibodies against intra-acrosomal proteins. Besidesdiagnostics of sperm pathology, monoclonal antibodies against intra-acrosomal proteinscan be used for evaluation of the physiological state of sperm cells as well as for selectionof a suitable method of fertilization in the laboratories of assisted reproduction.
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Specificity
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The antibody Hs-14 reacts with a 220 kDa testis-specific human intra-acrosomal proteinassociated with the membranes of the acrosomal vesicle. Species: Human and Mouse. Others not tested.
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Application Notes
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Suitable for Flow Cytometry analysis of sperm cells using 20 μl reagent / 100 μl cellsuspension. The content of a vial (1 ml) is sufficient for 50 tests. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
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Buffer
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Tris buffered saline (TBS) containing 15 mM Sodium Azide as preservativeand 0.2% (w/v) high-grade BSA (protease free) as stabilizer.Label: Fluorescein isothiocyanate (FITC)
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Storage
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Store the antibody in the dark at 2-8°C. DO NOT FREEZE! Avoid prolonged exposure to light. Shelf life: One year from despatch.
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Restrictions
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For Research Use only
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Alternatives for antigen "Acrosomal Vesicle Protein 1 (ACRV1)", type "Antibodies"
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Hosts
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Mouse (19), Rabbit (2)
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Reactivities
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Human (13), Dog (Canine) (4), Cow (Bovine) (2), Pig (Porcine) (2), Mouse (Murine) (1)
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Applications
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Western Blotting (WB) (21), Immunofluorescence (IF) (11), Immunocytochemistry (ICC) (7), Flow Cytometry (FACS) (5), ELISA (Detection) (2), ELISA (1)
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Alternatives 
