Acrosomal Vesicle Protein 1 (ACRV1) antibody

Details for Product No. ABIN93928
Antigen
Reactivity
Dog (Canine)
51
7
4
2
2
1
1
Host
Mouse
34
24
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
4
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Western Blotting (WB)
36
15
13
12
7
6
4
3
2
1
1
1
Options
Immunogen Freshly ejaculated dog sperms were washed in PBS and extracted in 3% acetic acid, 10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0.2% acetic acid, lyophylized and subsequently used for immunization.
Clone Ds-2
Isotype IgG
Specificity The antibody Ds-2 reacts with canine intra-acrosomal sperm protein (92 and 42 kDa), a testis-specific acrosomal vesicle protein 1 associated with the acrosomal membranes and matrix of the mature sperm.
Cross-Reactivity (Details) Canine (Dog)
Purification Purified by protein-A affinity chromatography
Purity > 95 % (by SDS-PAGE)
Antigen
Alternative Name Intra-Acrosomal Protein (ACRV1 Antibody Abstract)
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Product cited in: Geussová, P?knicová, Capková, Kaláb, Moos, Philimonenko, Hozák: "Monoclonal antibodies to canine intra-acrosomal sperm proteins recognizing acrosomal status during capacitation and acrosome reaction." in: Andrologia, Vol. 29, Issue 5, pp. 261-8, 1997 (PubMed).