Mouse anti-Cow (Bovine) IgG Antibody

Details for Product No. ABIN238423, Supplier: Log in to see
Antigen
Reactivity
Cow (Bovine)
2780
2760
2347
1724
1436
778
607
425
364
338
330
324
307
290
169
148
126
114
41
29
27
25
12
11
9
8
6
5
5
4
4
4
4
4
4
4
4
3
3
3
2
2
2
1
1
1
1
1
Host
Mouse
6750
4661
1834
811
684
394
88
52
51
9
8
5
5
4
2
1
1
Clonality (Clone)
Monoclonal ()
Application
Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
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Immunogen Fraction of Bovine immunoglobulin.
Clone IVA285-1
Isotype IgG1
Cross-Reactivity (Details) Species reactivity (tested):Bovine.
Purification Protein A affinity chromatography
Background Synonyms: Bovine Immunoglobulin G
Research Area Immunology, Secondary Antibodies
Application Notes Western Blotting (Reducing conditions): 0,5-1 μg/mL, overnight in 4C. SDS-PAGE (12 % separating gel). Positive control and sample preparation: BS (bovine serum), dilution 1:50 in Laemmlireducing buffer, boiled in water bath for 3 min. Strongly reacts with bovine IgG light chains, weakly reacts with IgM. Flow Cytometry. Immunohistochemistry (frozen sections).

Other applications not tested.
Otimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer PBS, pH 7.4, 15 mM Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -28 °C for longer.
Supplier Images
Western Blotting (WB) image for Mouse anti-Cow (Bovine) IgG Antibody (ABIN238423) Mouse anti-Cow (Bovine) IgG antibody
Background publications Antalíková, Simon, Jankovicová, Horovská, Dusinský, Hluchý: "Monoclonal antibody to the light chain of bovine immunoglobulin." in: Hybridoma (2005), Vol. 25, Issue 5, pp. 309-12, 2006 (PubMed).