Fucosyltransferase 4 (Alpha (1,3) Fucosyltransferase, Myeloid-Specific) (FUT4) antibody (PE)

Antigen: Fucosyltransferase 4 (Alpha (1,3) Fucosyltransferase, Myeloid-Specific) (FUT4)

Synonyms: LeX, CD15, ELFT, FCT3A, FUTIV, SSEA-1, FUC-TIV

Clonality: Monoclonal (MEM-158)

Host: Mouse

Reactivity: Human

Conjugate: PE

Application: Flow Cytometry (FACS)

Catalog no.: ABIN238450

Additional Information

Alternative name: CD15

Swiss-Prot: P22083

Immunogen: Human granulocytes

Cross-Reactivity: Human

Isotype: IgM

Clone: MEM-158

Description: CD15 (Lewis X, Le(x), stage specific embryonic antigen-1, SSEA-1) is a trisacharide determinant (3-fucosyl-N-acetyllactosamine) expressed on several glycolipids, glycoproteins and proteoglycans of various cell types, e.g. granulocytes, mast cells, monocytes, macrophages, cells of gastric mucosa, nervous system or various tumour cells. There are several variants of Lewis x, such as sialyl-Lewis x or sulphated Lewis x. Cells with high surface expression of Le(x) antigen exhibit strong self-aggregation, based on calcium-dependent Le(x)-Le(x) interaction. This process is involved for example in embryo compaction or in autoaggregation of teratocarcinoma cells. Sialyl-Le(x) and its isomer sialyl-Le(a) are ligands of selectins. CD15 expression has been extensively used to confirm diagnosis of Hodgkin´s disease. Alternate Names: Lewis x, SSEA-1.

Specificity: The antibody MEM-158 reacts with CD15, a cell membrane molecule 3-fucosyl-N- acetyllactosamine (3-FAL) strongly expressed on granulocytes, monocytes, macrophages, mast cells, it is also present on Langerhans cells and some myeloid precursors cells. Negative.

Application Details

Application Notes: Suitable for Flow Cytometry analysis of human blood cells using 20 m l reagent / 100 m l of whole blood or 10e6 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Buffer: PBS containing 15 mM sodium azide as preservative and 0.2% (w/v) high-grade BSA (Protease free) as stabilizer.

Storage: Store the antibody in the dark at 2-8°C. Do Not Freeze! Avoid prolonged exposure to light. Shelf Life: one year from despatch.

Research Area: Stem Cells, Hematopoietic Progenitors, Hematopoietic Stem Cells, CD Antigens, Surface Receptors of Immune Cells

Restrictions: For Research Use only