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CD4 Molecule (CD4) antibody
| Antigen | CD4 Molecule (CD4) |
| Synonyms | CD4, MGC165891, cd4, CD4mut, L3T4, Ly-4, p55, W3/25 |
| Clonality | Monoclonal (MEM-16) |
| Host |
 Alternatives Mouse |
| Reactivity |
Alternatives Human |
| Application |
Alternatives Flow Cytometry (FACS), Immunoprecipitation (IP)
|
| Catalog no. | ABIN238451 |
| Quantity | 0.1 mg (1.0 mg/ml) |
| Price | 265.00 $ Plus shipping costs $35.00 |
| Shipping to |
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| Availability | Ships within 7 to 10 Business Days |
Additional Information
| Characteristics | Synonyms: T4/Leu-3, T-cell surface glycoprotein CD4, T-cell surface antigen T4/Leu-3 |
| Alternative name | CD4 |
| Swiss-Prot | P01730 |
| Immunogen | HPB cell line (human peripheral blood leukemia T-cells). |
| Cross-Reactivity | Human |
| Isotype | IgM (Matching secondary antibodies) |
| Clone | MEM-16 |
| Description | CD4 is a single chain transmembrane glycoprotein and belongs to immunoglobulinsupergene family. In extracellular region there are 4 immunoglobulin-like domains (1 Ig-likeV-type and 3 Ig-like C2-type). Transmembrane region forms 25 aa, cytoplasmic tail consistsof 38 aa. Domains 1,2 and 4 are stabilized by disulfide bonds. The intracellular domain ofCD4 is associated with p56Lck, a Src-like protein tyrosine kinase. It was described that CD4segregates into specific detergent-resistant T-cell membrane microdomains. Extracellular ligands: MHC class II molecules (binds to CDR2-like region in CD4 domain 1),HIV envelope protein gp120 (binds to CDR2-like region in CD4 domain 1), IL-16 (binds toCD4 domain 3), Human seminal plasma glycoprotein gp17 (binds to CD4 domain 1),L-selectinIntracellular ligands: p56LckCD4 is a co-receptor involved in immune response (co-receptor activity in binding to MHCclass II molecules) and HIV infection (human immunodeficiency virus, CD4 is primaryreceptor for HIV-1 surface glycoprotein gp120). CD4 regulates T-cell activation, T/B-celladhesion, T-cell diferentiation, T-cell selection and signal transduction. Defects in antigenpresentation (MHC class II) cause dysfunction of CD4+ T-cells and their almost completeabsence in patients blood, tissue and organs (SCID immunodeficiency). |
| Specificity | The antibody MEM-16 recognizes an epitope EF loop of D1 domain of CD4 antigen, a 55 kDatransmebrane glycoprotein expressed on a subset of T lymphocytes (''helper'' T-cells) andalso on monocytes, tissue macrophages and granulocytes. Negative Species: Porcine. |
Application Details
| Application Notes | Flow Cytometry: 10 μg/mlPositive control: Peripheral blood lymphocytesImmunoprecipitation. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user. |
| Concentration | 1.0 mg/ml |
| Purification | Purified |
| Buffer | PBS, pH 7.4 with 15 mM sodium azide as preservative. |
| Storage | Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
| Research Area | Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells |
| Restrictions | For Research Use only |
Alternatives
Alternatives for antigen "CD4 Molecule (CD4)", type "Antibodies"
