CD4 Molecule (CD4) antibody

Details for Product No. ABIN238451
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Antigen
Synonyms cd4, L3T4, Ly-4, CD4mut, W3/25, p55
Reactivity
Human
(674), (498), (143), (41), (31), (25), (24), (22), (20), (20), (17), (10), (6), (5), (4), (3), (2), (2), (1)
Host
Mouse
(737), (433), (180), (3), (1)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(192), (169), (143), (72), (17), (15), (12), (10), (10), (9), (9), (8), (6), (6), (6), (6), (5), (5), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Flow Cytometry (FACS), Immunoprecipitation (IP)
(1008), (243), (231), (173), (164), (152), (101), (97), (59), (53), (30), (21), (12), (7), (5), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Pubmed 4 references available
Quantity 0.1 mg
Shipping to United States (Change)
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Catalog No. ABIN238451
308.00 $
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Immunogen HPB cell line (human peripheral blood leukemia T-cells).
Clone MEM-16
Isotype IgM
Specificity The antibody MEM-16 recognizes an epitope EF loop of D1 domain of CD4 antigen, a 55 kDa transmebrane glycoprotein expressed on a subset of T lymphocytes (''helper'' T-cells) and also on monocytes, tissue macrophages and granulocytes.
Characteristics Synonyms: T4/Leu-3, T-cell surface glycoprotein CD4, T-cell surface antigen T4/Leu-3
Purification Precipitation methods and size-exclusion chromatography.
Alternative Name CD4
Background CD4 is a single chain transmembrane glycoprotein and belongs to immunoglobulin supergene family. In extracellular region there are 4 immunoglobulin-like domains (1 Ig-like V-type and 3 Ig-like C2-type). Transmembrane region forms 25 aa, cytoplasmic tail consists of 38 aa. Domains 1,2 and 4 are stabilized by disulfide bonds. The intracellular domain of CD4 is associated with p56Lck, a Src-like protein tyrosine kinase. It was described that CD4 segregates into specific detergent-resistant T-cell membrane microdomains. Extracellular ligands: MHC class II molecules (binds to CDR2-like region in CD4 domain 1), HIV envelope protein gp120 (binds to CDR2-like region in CD4 domain 1), IL-16 (binds to CD4 domain 3), Human seminal plasma glycoprotein gp17 (binds to CD4 domain 1), L-selectin Intracellular ligands: p56Lck CD4 is a co-receptor involved in immune response (co-receptor activity in binding to MHC class II molecules) and HIV infection (human immunodeficiency virus, CD4 is primary receptor for HIV-1 surface glycoprotein gp120). CD4 regulates T-cell activation, T/B-cell adhesion, T-cell diferentiation, T-cell selection and signal transduction. Defects in antigen presentation (MHC class II) cause dysfunction of CD4+ T-cells and their almost complete absence in patients blood, tissue and organs (SCID immunodeficiency).Synonyms: T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4
Gene ID 920
UniProt P01730
Research Area Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells
Application Notes Flow Cytometry: 10 μg/mLPositive control: Peripheral blood lymphocytesImmunoprecipitation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer PBS, pH 7.4 with 15 mM sodium azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Expiry Date 12 months
Background publications Millán, Cerny, Horejsi et al.: "CD4 segregates into specific detergent-resistant T-cell membrane microdomains." in: Tissue antigens, Vol. 53, Issue 1, pp. 33-40, 1999 (PubMed).

Foti, Phelouzat, Holm et al.: "p56Lck anchors CD4 to distinct microdomains on microvilli." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 4, pp. 2008-13, 2002 (PubMed).

Clapham, McKnight: "Cell surface receptors, virus entry and tropism of primate lentiviruses." in: The Journal of general virology, Vol. 83, Issue Pt 8, pp. 1809-29, 2002 (PubMed).

Brdicková, Brdicka, Angelisová et al.: "LIME: a new membrane Raft-associated adaptor protein involved in CD4 and CD8 coreceptor signaling." in: The Journal of experimental medicine, Vol. 198, Issue 10, pp. 1453-62, 2003 (PubMed).

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