Endoglin (ENG) antibody
| Antigen |
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| Synonyms |
END, ORW, HHT1, ORW1, CD105, FLJ41744, AI528660, AI662476, S-endoglin, Eng, MGC124909, ENG, MGC137842, DKFZp469D0419 |
| Clonality |
Monoclonal (MEM-229) |
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Host
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Reactivity
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Application
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 Alternatives Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF)
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| Catalog no. |
ABIN238459 |
| Quantity |
0.1 mg (1.0 mg/ml) |
| Price |
265.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 7 to 10 Business Days |
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Characteristics
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Synonyms: ENG, END, HHT1, ORW, ORW1
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Alternative name
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CD105 / Endoglin
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Swiss-Prot
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P17813
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Immunogen
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Recombinant Vaccinia virus containing the Human CD105 (L-isoform) cDNA.
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Cross-Reactivity
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Human, Pig (Porcine)
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Isotype
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IgG2a (Matching secondary antibodies)
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Clone
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MEM-229
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Description
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CD105 (Endoglin) is a homodimeric transmembrane glycoprotein serving in presence ofTGFβR-2 as a receptor for TGFβ-1 and TGFβ-3. CD105 is highly expressed on endothelial cellsand promotes angiogenesis during wound healing, infarcts and in a wide range of tumoursand its gene expression is stimulated by hypoxia. CD105 prevents apoptosis in hypoxicendothelial cells and also antagonises the inhibitory effects of TGFβ-1 on vascularendothelial cell growth and migration. Normal cellular levels of CD105 are required forformation of new blood vessels.
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Specificity
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The antibody MEM-229 recognizes CD105 (Endoglin), a 180 kDa type I integral membranehomodimer glycoprotein expressed on vascular endothelial cells (small and large vessels),activated monocytes and tissue macrophages, stromal cells of certain tissues includingbone marrow, pre-B lymphocytes in fetal marrow and erythroid precursors in fetal andadult bone marrow, it is also present on syncytiotrophoblast on placenta throughoutpregnancy. Species: Human, Porcine. Others not tested.
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Application Notes
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Flow Cytometry: 10 μg/mlPositive Control: Kg1 Human acute myelogenous leukemia cell lineWestern Blotting (Non-Reducing Conditions). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
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Concentration
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1.0 mg/ml
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Purification
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Purified
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Buffer
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PBS, pH~7.4 with 15 mM Sodium Azide as preservative.
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Storage
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Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
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Research Area
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CD Antigens, Surface Receptors of Immune Cells
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Restrictions
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For Research Use only
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Alternatives for antigen "Endoglin (ENG)", type "Antibodies"
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Hosts
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Mouse (48), Rabbit (21), Rat (6)
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Reactivities
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Human (59), Mouse (Murine) (11), Horse (Equine) (6), Pig (Porcine) (5), Rat (Rattus) (1)
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Applications
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Flow Cytometry (FACS) (57), Western Blotting (WB) (44), Immunoprecipitation (IP) (20), Immunohistochemistry (Frozen Sections) (IHC (fro)) (13), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (10), Enzyme Immunoassay (EIA) (9), Immunohistochemistry (IHC) (7), ELISA (5), ELISA (Detection) (3), Immunofluorescence (IF) (2)
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Conjugates
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FITC (8), PE (7), APC (2), Biotin (2), Alexa Fluor 488 (1), Alexa Fluor 700 (1), DY647 (1), Dyomics 647 (1), Pacific Blue (1)
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Epitopes
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AA 26-176 (4), Center E395 (1)
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