HLA Class II DR beta 1 (HLA-DRB1) antibody

Antigen: HLA Class II DR beta 1 (HLA-DRB1)

Synonyms: SS1, DRB1, DRw10, HLA-DRB, FLJ75017, FLJ76359, HLA-DR1B, HLA-DRB1*, HLA-DR3B, HLA-DR52, MGC117330, MGC108235

Clonality: Monoclonal (MEM-267)

Host: Mouse

Reactivity: Human

Application: Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Western Blotting (WB)

Catalog no.: ABIN238469

Additional Information

Characteristics: Synonyms: MHC class II antigen DRB1, DR1, DR-1, HLA class II histocompatibility antigen, DRB1-1 betachain

Alternative name: HLA class II DR beta 1 / HLA-DRB1

Swiss-Prot: P04229

Immunogen: Purified, insoluble DR1 beta chain (DRB1*0101) expressed in E. coli inclusion bodies.

Cross-Reactivity: Human

Isotype: IgG2b  (Matching secondary antibodies)

Clone: MEM-267

Description: HLA-DR1 belongs to the HLA class II beta chain paralogues. The MHC Class II molecule is aheterodimer consisting of an alpha (DRA) and a beta chain (DRB), both anchored in themembrane. It plays a central role in the immune system by presenting peptides derivedfrom extracellular proteins. MHC Class II molecules are expressed in antigen presentingcells (APC). The beta chain is approximately 26-28 kDa. Within the DR molecule the betachain contains all the polymorphisms specifying the peptide binding specificities. Hundreds of DRB1 alleles have been described and typing for these polymorphisms isroutinely done for bone marrow and kidney transplantation.

Specificity: The antibody MEM-267 specifically binds to the empty but not peptide-loaded form ofHLA-DR1. DR is the isotypes of human MHC Class II molecules expressed onantigen-presenting cells (APC, dendritic cells, B lymphocytes, monocytes, macrophages). Species: Human. Others not tested.

Application Details

Application Notes: Western Blotting. Flow Cytometry: The antibody MEM-267 stains immature dendritic cells that express emptycell surface MHC molecules, but not cells that express predominantly peptide loadedforms. ELISA. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 1.0 mg/ml

Purification: Aff - Purified

Buffer: PBS, pH 7.4 containing 15 mM sodium azide as preservative.

Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Restrictions: For Research Use only