HLA-DRB1 antibody (AA 27-264)

Catalog No. ABIN7440767

The Rabbit Polyclonal anti-HLA-DRB1 antibody is suitable to detect HLA-DRB1 in samples from Mouse. It has been validated for WB, IHC, IP and ICC.

Quick Overview for HLA-DRB1 antibody (AA 27-264) (ABIN7440767)

Target: HLA-DRB1 (Major Histocompatibility Complex, Class II, DR beta 1 (HLA-DRB1))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HLA-DRB1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-HLA-DRB1 Antibody

Binding Specificity: AA 27-264

Purpose: Polyclonal Antibody to HLA Class II Histocompatibility Antigen, DRB1 Beta Chain (HLA-DRB1)

Specificity: The antibody is a rabbit polyclonal antibody raised against HLA-DRB1. It has been selected for its ability to recognize HLA-DRB1 in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant HLA Class II Histocompatibility Antigen, DRB1 Beta Chain (HLA-DRB1) corresdonding to Val27~Ser264

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL,Immunocytochemistry in formalin fixed cells: 5-20 μg/mL,Immunohistochemistry in formalin fixed frozen section: 5-20 μg/mL,Immunohistochemistry in paraffin section: 5-20 μg/mL,Enzyme-linked Immunosorbent Assay: 0.05-2 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for HLA-DRB1

Target: HLA-DRB1 (Major Histocompatibility Complex, Class II, DR beta 1 (HLA-DRB1))

Alternative Name: HLA-DRB1

Background: HLA-DRB1, MHCDRb1, HLADRb1, Major Histocompatibility Complex Class II DR Beta 1, HLA Class II Histocompatibility Antigen,DRB1-9 Beta Chain, HLA class II histocompatibility antigen, DRB1-15 beta chain

UniProt: P01915

Pathways: TCR Signaling, Positive Regulation of Peptide Hormone Secretion, Production of Molecular Mediator of Immune Response, CXCR4-mediated Signaling Events, Cancer Immune Checkpoints, Human Leukocyte Antigen (HLA) in Adaptive Immune Response