HLA Class II DR beta 1 (HLA-DRB1) antibody (PE)

Antigen: HLA Class II DR beta 1 (HLA-DRB1)

Synonyms: SS1, DRB1, DRw10, HLA-DRB, FLJ75017, FLJ76359, HLA-DR1B, HLA-DRB1*, HLA-DR3B, HLA-DR52, MGC117330, MGC108235

Clonality: Monoclonal (MEM-267)

Host: Mouse

Reactivity: Human

Conjugate: PE

Application: Flow Cytometry (FACS)

Catalog no.: ABIN238470

Additional Information

Characteristics: Synonyms: MHC class II antigen DRB1, DR1, DR-1, HLA class II histocompatibility antigen, DRB1-1 betachain

Alternative name: HLA class II DR beta 1 / HLA-DRB1

Swiss-Prot: P04229

Immunogen: Purified, insoluble DR1 beta chain (DRB1*0101) expressed in E. coli inclusion bodies.

Cross-Reactivity: Human

Isotype: IgG2b

Clone: MEM-267

Description: HLA-DR1 belongs to the HLA class II beta chain paralogues. The MHC Class II molecule is aheterodimer consisting of an alpha (DRA) and a beta chain (DRB), both anchored in themembrane. It plays a central role in the immune system by presenting peptides derivedfrom extracellular proteins. MHC Class II molecules are expressed in antigen presentingcells (APC). The beta chain is approximately 26-28 kDa. Within the DR molecule the betachain contains all the polymorphisms specifying the peptide binding specificities. Hundreds of DRB1 alleles have been described and typing for these polymorphisms isroutinely done for bone marrow and kidney transplantation.

Specificity: Reacts with immature dendritic cells that express empty cell surface MHC molecules, butnot cells that express predominantly peptide loaded forms, reacts specifically with theempty but not peptide-loaded form of HLA-DR1. This antibody specifically binds to the empty but not peptide-loaded form of HLA-DR1. DR isthe isotypes of human MHC Class II molecules expressed on antigen-presenting cells (APC,dendritic cells, B lymphocytes, monocytes, macrophages). Species: Human. Others not tested.

Application Details

Application Notes: Suitable for Flow Cytometry analysis: 5 µg/ml. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 1.0 mg/ml

Buffer: PBS containing 15 mM Sodium Azide as preservative and 0.2% (w/v)high-grade BSA (Protease free) as stabilizer.Label: Conjugated with R-Phycoerythrin (PE) under optimum conditions.

Storage: Store the antibody in the dark at 2-8°C. DO NOT FREEZE! Avoid prolonged exposure to light. Shelf life: one year from despatch.

Restrictions: For Research Use only