Transferrin Receptor (p90, CD71) (TFRC) antibody (PE)

Antigen: Transferrin Receptor (p90, CD71) (TFRC)

Synonyms: TFR, CD71, TFR1, TRFR, TR, p90, Trfr, Mtvr1, Mtvr-1, AI195355, AI426448, AU015758, 2610028K12Rik, E430033M20Rik, TFRC, tfr, cd71, tfr1, trfr, DKFZp469M0811, LOC100219280

Clonality: Monoclonal (MEM-75)

Host: Mouse

Reactivity: Human

Conjugate: PE

Application: Flow Cytometry (FACS)

Catalog no.: ABIN238476

Additional Information

Alternative name: CD71 / TFRC

Swiss-Prot: P02786

Immunogen: NALM-6 human pre-B cell line

Cross-Reactivity: Human

Isotype: IgG1

Clone: MEM-75

Description: CD71 (transferrin receptor) is a type II transmembrane glycoprotein expressed as homodimer in erythroid blood cell line and in activated leukocytes. Upon binding of holotransferrin (complex of transferrin and iron ions), CD71 is internalized by clathrin-mediated endocytosis. Acidification of endosomes by vesicular membrane proton pumps leads to dissociation of iron ions, whereas transferrin (apotransferrin) remains associated with CD71 and recycles to the cell surface, where it is released upon exposure to normal pH. CD71 is also involved in uptake of non-transferrin bound iron.

Specificity: The antibody MEM-75 reacts with CD71 antigen (transferrin receptor), a 95 kDa type II homodimeric transmembrane glycoprotein expressed on activated B and lymphocytes, macrophages and erythroid precursors, it is lost on resting blood leukocytes. The antibody MEM-75 does not block binding of transferrin to the receptor.

Application Details

Application Notes: Further Comments: This antibody is designed for Flow Cytometry analysis of human blood cells using 20 m l reagent/100 m l of whole blood or 10e6 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.

Buffer: PBS containing 15 mM sodium azide as preservative and 0.2% (w/v) high-grade BSA (Protease free) as stabilizer.

Storage: Store the antibody in the dark at 2-8°C. Do Not Freeze! Avoid prolonged exposure to light. Shelf Life: one year from despatch.

Research Area: CD Antigens, Surface Receptors of Immune Cells

Restrictions: For Research Use only