Transferrin Receptor antibody (AA 1-198)

Catalog No. ABIN3043538

There is 1 publication for this product available. The Rabbit Polyclonal anti-Transferrin Receptor antibody is suitable to detect Transferrin Receptor in samples from Human, Mouse and Rat. It has been validated for FACS, WB, IHC, IF, ICC and IHC (fro).

Quick Overview for Transferrin Receptor antibody (AA 1-198) (ABIN3043538)

Target: Transferrin Receptor (TFRC) (Transferrin Receptor (p90, CD71) (TFRC))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Transferrin Receptor antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-Transferrin Receptor Antibody

Binding Specificity: AA 1-198

Purpose: Anti-Transferrin Receptor/TFRC Antibody

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: Human TFRC shares 72%,70% amino acid (aa) sequences identity with mouse,rat TFRC,respectively.

Characteristics: Anti-Transferrin Receptor/TFRC Antibody. Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human TFRC recombinant protein (Position: M1-N198). Human TFRC shares 72% and 70% amino acid (aa) sequences identity with mouse and rat TFRC, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human Immunohistochemistry (Frozen Section), 0.5-1 μg/mL, Human Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human Immunofluorescence, 5 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

References for anti-Transferrin Receptor antibody (ABIN3043538)

Li, Wang, Ma, Yang, Zhang, Dawe, Xiao: "Amyloid precursor protein modulates Nav1.6 sodium channel currents through a Go-coupled JNK pathway." in: Scientific reports, Vol. 6, pp. 39320, (2018) (PubMed).

Target Details for Transferrin Receptor

Target: Transferrin Receptor (TFRC) (Transferrin Receptor (p90, CD71) (TFRC))

Alternative Name: TFRC

Background:

Background: Transferrin receptor protein 1 (TfR1), also known as Cluster of Differentiation 71 (CD71), is a protein that in humans is encoded by the TFRC gene. It is mapped to 3q29. TFRC is a transmembrane glycoprotein composed of two disulfide-linked monomers joined by two disulfide bonds. Expression of human TFR1 in hamster cell lines markedly enhanced the infection of viruses pseudotyped with the glycoprotein of Machupo, Guanarito, and Junin viruses. TFR1 is a cellular receptor for New World hemorrhagic fever arenaviruses. It is required for iron delivery from transferrin to cells.

Gene Full Name: transferrin receptor

Sequence Similarities: Belongs to the peptidase M28 family. M28B subfamily.

Molecular Weight: 95 kDa

Gene ID: 7037

UniProt: P02786

Pathways: Transition Metal Ion Homeostasis