SOS1 antibody

Antigen: SOS1

Clonality: Monoclonal (SOS-1)

Host: Mouse

Reactivity: Human

Application: Enzyme Immunoassay (EIA), Immunofluorescence (IF), Western Blotting (WB)

Catalog no.: ABIN238504

Additional Information

Characteristics: Synonyms: SOS-1, Son of sevenless homolog 1

Gene ID: 6654

Swiss-Prot: Q07889

Immunogen: Peptide corresponding to amino acids THPSMHRDGPPLLENAHSS of human Sos protein.

Cross-Reactivity: Human, Mouse (Murine)

Isotype: IgG1  (Matching secondary antibodies)

Clone: SOS-1

Description: The guanine nucleotide exchange factor Sos (Son-of-sevenless) is a complex multidomainprotein that activates the small GTPase Ras (H-Ras, K-Ras, N-Ras, but not functionallydistinct R-Ras) in response to receptor tyrosine kinase stimulation. Nucleotide exchangeactivity of Sos is stimulated by allosteric Ras binding. By another (separable) guanineexchange factor domain domain Sos modulates activity of Rac/Rho GTPases. Sos thusintegrates signals that affect both gene expression and cytoskeletal reorganization, theSos-mediated Ras-activation and Rac activation differ in composition and stability of theformed complex.

Specificity: The antibody SOS-01 reacts with human Sos, an ubiquitously expressed 150 kDaintracellular protein. Species: Human and mouse. Others not tested.

Application Details

Application Notes: Western Blotting under reducing conditions (1 µg/ml): Positive control: HeLa human cervix carcinoma cell line. Immunocytochemistry. ELISA. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 1.0 mg/ml

Purification: Aff - Purified

Buffer: PBS, pH 7.4 with 15 mM sodium azide as preservative.

Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid freeze/thaw cycles. Shelf life: One year from despatch.

Research Area: Cancer

Restrictions: For Research Use only