SOS1 antibody (AA 1243-1258)

Catalog No. ABIN210638

Product Details anti-SOS1 Antibody

Target: SOS1 (Son of Sevenless Homolog 1 (SOS1))

Binding Specificity: AA 1243-1258

Reactivity: Human, Mouse, Rabbit, Horse, Dog, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SOS1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Specificity: Recognizes SOS 1, Mr 175kD. Species cross-reactivity: Mouse and human. Other species not tested.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Elephant, Panda, Dog, Horse, Rabbit (100%) Bovine, Bat, Pig (94%) Opossum (88%).

Purification: Protein A purified

Immunogen: KLH-conjugated, synthetic peptide corresponding to amino acids 1243-1258 (KKSDHGNAFFPNSPSP) of murine Son of Sevenless Homolog 1 Protein (SOS 1). The immunizing sequence is identical in human. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Elephant, Panda, Dog, Horse, Rabbit (100%), Bovine, Bat, Pig (94%), Opossum (88%).

Type of Immunogen: Synthetic peptide - KLH conjugated

Isotype: IgG

Application Details

Application Notes: Approved: IP, WB (0.5 - 1 μg/mL)

Usage: Suitable for use in Western Blot and Immunoprecipitation. Western Blot: 0.5-1 μg/mL. Immunoprecipitation: 5 μg. Positive control: F4209: Fibroblast Cell, 3T3/A31. Add 2.5 μL of 2-mercaptoethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.1 M Tris,  pH 8.0, 0.15 M sodium chloride, 0.1 mM EDTA, 0.05 % sodium azide, 30 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles.

Target Details for SOS1

Target: SOS1 (Son of Sevenless Homolog 1 (SOS1))

Alternative Name: SOS1 / HGF (SOS1 Products)

Synonyms: GF1 antibody, GGF1 antibody, GINGF antibody, HGF antibody, NS4 antibody, 4430401P03Rik antibody, 9630010N06 antibody, AI449023 antibody, SOS Ras/Rac guanine nucleotide exchange factor 1 antibody, son of sevenless homolog 1 (Drosophila) antibody, SOS1 antibody, Sos1 antibody

Target Type: Viral Protein

Background: Name/Gene ID: SOS1

Synonyms: SOS1, GINGF, NS4, SOS-1, Son of sevenless homolog 1, GF1, GGF1

Gene ID: 6654

UniProt: Q07889

Pathways: RTK Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Hepatitis C, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, BCR Signaling