zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) antibody

Antigen: zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70)

Synonyms: SRK, STD, TZK, ZAP-70, FLJ17670, FLJ17679, Srk, mur, 70kDa, mrtle, AI327364, MGC108902, zap70, MGC80670, MGC88962, ZAP70

Clonality: Monoclonal (ZAP-03)

Host: Mouse

Reactivity: Human

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Western Blotting (WB)

Catalog no.: ABIN238530

Additional Information

Characteristics: Synonyms: ZAP-70, ZAP 70, SRK, 70 kDa zeta-associated protein, Syk-related tyrosine kinase

Alternative name: ZAP70

Swiss-Prot: P43403

Immunogen: Bacterially expressed fusion protein representing C-terminal part (160 amino acids) ofhuman ZAP-70.

Cross-Reactivity: Human

Isotype: IgG1  (Matching secondary antibodies)

Clone: ZAP-03

Description: The ZAP70 (zeta-associated protein of 70 kDa) tyrosine kinase was identified as a tyrosinephosphoprotein that associates with TCR zeta subunit and undergoes tyrosinephosphorylation following TCR stimulation. ZAP70 is a Syk family tyrosine kinase primarilyexpressed in T and NK cells that plays an essential role in signaling through the TCR. TCR-mediated activation of T cells is crucial to the immune response. In humans, ZAP70gene mutations resulting in lower ZAP70 protein expression levels or expression ofcatalytically inactive ZAP70 proteins, have been identified. ZAP70 deficiency results in theabsence of mature CD8+ T cells and the prevention of TCR-mediated activation of CD4+ Tcells, and it can lead to severe combined immunodeficiency. In patients with chroniclymphocytic leukemia (B-CLL), ZAP70 expression on B cell was shown to be correlated withdisease progression and survival. ZAP70 is cytosolic protein migrating at 70 kDa in SDS-PAGE. It contains two N-terminal SH2domains (Src homology domain 2) and a C-terminal kinase domain. Crystal structure of theZAP70 SH2 domains in complex with a TCR zeta subunit peptide was described. During Tcell activation , the binding of ZAP70 SH2 domains to the phosphorylated zeta subunit onthe activated TCR complex causes a colocalization with the Lck tyrosine kinase thatphosphorylates ZAP70 on Tyr493 in the activation loop. ZAP70 autophosphorylatesmultiple tyrosines in the region between the SH2 domains and the kinase domain,including the binding sites for additional SH2-containing signaling proteins such as SLP76,LAT, Lck, PLCgamma1, Vav, Shc, Ras-GAP, and Abl. ZAP70-mediated activation of thesedownstream effectors leads to the release of intracellular calcium stores, and thetranscription of interleukin-2 and other genes important for an immune response.

Specificity: The antibody ZAP-03 reacts with ZAP70, a 70 kDa protein tyrosine kinase expressed in Tand NK cells. ZAP70 is a molecule susceptible to degradation. It is recommended to use freshlyprepared cell lysates (protease inhibitors are essential) to avoid non-specific staining ofdegradation products. Species: Human. Others not tested.

Application Details

Application Notes: Flow Cytometry (2-5 μg/ml): Intracellular staining. Positive control: HPB-ALL human peripheral blood T cell leukemia cell line.

Concentration: 1.0 mg/ml

Purification: Aff - Purified

Buffer: PBS, pH 7.4 containing 15 mM sodium azide as preservative.

Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Research Area: Signaling, Tags/Labels, Immunology, Cancer, CD Antigens, Surface Receptors of Immune Cells

Restrictions: For Research Use only