Lipopolysaccharide-Induced Tumor Necrosis Factor-alpha Factor (LITAF) antibody

Antigen: Lipopolysaccharide-Induced Tumor Necrosis Factor-alpha Factor (LITAF)

Synonyms: PIG7, SIMPLE, TP53I7, FLJ38636, MGC116698, MGC116700, MGC116701, MGC125274, MGC125275, MGC125276

Clonality: Polyclonal

Host: Goat

Reactivity: Please enquire

Application: ELISA

Catalog no.: ABIN238579

Additional Information

Alternative name: LITAF

Gene ID: 9516, 56722 (mouse), 65161 (rat)

Immunogen: C-PDGKGMNPPSYYTQ

Description: All names and symbols: LITAF, lipopolysaccharide-induced TNF factor, CMT1C, FLJ38636, MGC116698, MGC116700, MGC116701, MGC125274, MGC125275, MGC125276, PIG7, SIMPLE, TP53I7, LPS-induced TNF-alpha factor, small integral membrane protein of lysosome/late endosome, tumor protein. Official Symbol: LITAF. RefSeq number(s): NP_004853.2

Specificity: Human, Mouse, Rat

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:8000. Western Blot: Preliminary experiments gave an approx 25kDa band in human lymph node lysates after 0.1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 17.1kDa according to NP_004853.2. The 25kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Chromatin and Nuclear Signaling, Transcription Factors

Restrictions: For Research Use only

Publications

Tang, Metzger, Leeman et al.: "LPS-induced TNF-alpha factor (LITAF)-deficient mice express reduced LPS-induced cytokine: Evidence for LITAF-dependent LPS signaling pathways." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, Issue 37, pp. 13777-82, 2006 (PubMed).