Transmembrane Protease, Serine 3 (TMPRSS3) antibody

Antigen: Transmembrane Protease, Serine 3 (TMPRSS3)

Synonyms: DFNB8, DFNB10, ECHOS1, TADG12, MGC130589, TMPRSS3, DKFZp469L1528

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN238616

Additional Information

Alternative name: TMPRSS3

Gene ID: 64699, 140765 (mouse)

Immunogen: C-EKIVYHSKYKPKR

Format: Affinity-purified

Description: All names and symbols: transmembrane protease, serine 3, DFNB10, DFNB8, ECHOS1, TADG12, serine protease TADG12. Official Symbol: TMPRSS3. RefSeq number(s): NP_076927.1, NP_115777.1, NP_115780.1, NP_115781.1

Specificity: This antibody is expected to recognise all four reported isoforms (NP_076927.1, NP_115777.1, NP_115780.1, NP_115781.1) Human, Mouse

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Approx 48kDa band observed in A431 lysates (calculated MW of 49.4kDa according to NP_076927.1). Recommended concentration: 0.1-0.3µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Proteolysis / Ubiquitin

Restrictions: For Research Use only

Images

ABIN238616 (0.1 ug/ml) staining of A431 cell lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Lee, Park, Kim et al.: "Pathogenic mutations but not polymorphisms in congenital and childhood onset autosomal recessive deafness disrupt the proteolytic activity of TMPRSS3." in: Journal of medical genetics, Vol. 40, Issue 8, pp. 629-31, 2003 (PubMed).