Protein Phosphatase 2, Regulatory Subunit B'', alpha (PPP2R3A) antibody

Antigen: Protein Phosphatase 2, Regulatory Subunit B'', alpha (PPP2R3A)

Synonyms: PR72, PR130, PPP2R3, MGC29057, A730042E07, 3222402P14Rik, PPP2R3A, MGC152251

Clonality: Polyclonal

Host: Goat

Application: ELISA

Catalog no.: ABIN238630

Additional Information

Alternative name: PPP2R3A

Gene ID: 5523

Immunogen: C-QKDVENDGPEPSD

Format: Affinity-purified

Description: All names and symbols: PPP2R3A, protein phosphatase 2 (formerly 2A), regulatory subunit B'', alpha , PPP2R3, PR130, PR72, PP2A, subunit B, B''-PR72/PR130, PP2A, subunit B, B72/B130 isoforms, PP2A, subunit B, R3 isoform, Serine/threonine protein phosphatase 2A, 72/130 kDa regul. Official Symbol: PPP2R3A. RefSeq number(s): NP_002709.2, NP_871626.1

Specificity: This antibody is expected to recognise both reported isoforms (NP_002709.2, NP_871626.1). Human,

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: Preliminary experiments gave an approx 80kDa band in Human Skeletal Muscle lysates after 1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 130kDa according to NP_002709.2 and of 61.1kDa according to NP_871626.1. The 80kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Cancer, Cell Cycle, Kinases/Phosphatases

Restrictions: For Research Use only

Publications

Publications: Zhang, Cagatay, Amanai et al.: "Viable mice with compound mutations in the Wnt/Dvl pathway antagonists nkd1 and nkd2." in: Molecular and cellular biology, Vol. 27, Issue 12, pp. 4454-64, 2007 (PubMed).