Insulin-Like Growth Factor 2 MRNA Binding Protein 2 (IGF2BP2) antibody

Antigen: Insulin-Like Growth Factor 2 MRNA Binding Protein 2 (IGF2BP2)

Synonyms: p62, IMP2, IMP-2, VICKZ2, RGD1305614, IGF2BP2, DKFZp468I2113

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN238651

Additional Information

Alternative name: IGF2BP2

Gene ID: 10644, 319765 (mouse)

Immunogen: C-KQQEQKYPQGVASQR

Format: Affinity-purified

Description: All names and symbols: IGF2BP2, insulin-like growth factor 2 mRNA binding protein 2, IMP-2, IMP2, VICKZ2, p62, IGF II mRNA binding protein 2, IGF-II mRNA-binding protein 2. Official Symbol: IGF2BP2. RefSeq number(s): NP_006539.3, NP_001007226.1

Specificity: This antibody is expected to recognise both reported isoforms (NP_006539.3 and NP_001007226.1). Human, Mouse, Dog

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: Approx. 60-70kDa band observed in lysates of cell line HepG2 (calculated MW of 66.1kDa according to NP_006539.3). In transfected HEK293 transiently expressing IGF2BP2 a band of approx. 65kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 1-3µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Chromatin and Nuclear Signaling, Chromatin Binding Proteins, DNA/RNA

Restrictions: For Research Use only

Images

ABIN238651 (1µg/ml) staining of HepG2 cell lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

HEK293 overexpressing IGF2BP2 (RC205673) and probed with ABIN238651 (mock transfection in first lane), tested by Origene.

Publications

Publications: Zeggini, Weedon, Lindgren et al.: "Replication of genome-wide association signals in UK samples reveals risk loci for type 2 diabetes." in: Science (New York, N.Y.), Vol. 316, Issue 5829, pp. 1336-41, 2007 (PubMed).