Membrane-Associated Ring Finger (C3HC4) 1 (MARCH1) antibody

Antigen: Membrane-Associated Ring Finger (C3HC4) 1 (MARCH1)

Synonyms: RNF171, MARCH-I, FLJ20668, DKFZp564M1682, BB085186, 2900024D24Rik, RGD1305148, MARCH1, DKFZp459K1250

Clonality: Polyclonal

Host: Goat

Application: ELISA

Catalog no.: ABIN238660

Additional Information

Alternative name: MARCH-I

Gene ID: 55016, 72925 (mouse), 361135 (rat)

Immunogen: QSRLSVCPSTQD

Format: Affinity-purified

Description: All names and symbols: MARCH1, membrane-associated ring finger (C3HC4) 1, DKFZp564M1682, FLJ20668, MARCH-I, RNF171, membrane-associated RING-CH protein I. Official Symbol: 01.03.01. RefSeq number(s): NP_060393.1

Specificity: Human, Mouse, Rat

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Preliminary experiments in Human Colon, Ileum, Lung and Lymph Node lysates gave no specific signal but low background (at antibody concentration up to 1µg/ml). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates?

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Proteolysis / Ubiquitin, Adaptive Immunity

Restrictions: For Research Use only

Publications

Publications: Matsuki, Ohmura-Hoshino, Goto et al.: "Novel regulation of MHC class II function in B cells." in: The EMBO journal, Vol. 26, Issue 3, pp. 846-54, 2007 (PubMed).