MARCH1 antibody (C-Term)

Catalog No. ABIN6263124

The Rabbit Polyclonal anti-MARCH1 antibody is suitable to detect MARCH1 in samples from Human, Mouse and Rat. It has been validated for ELISA, WB, IF and ICC.

Quick Overview for MARCH1 antibody (C-Term) (ABIN6263124)

Target: MARCH1 (Membrane-Associated Ring Finger (C3HC4) 1, E3 Ubiquitin Protein Ligase (MARCH1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MARCH1 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-MARCH1 Antibody

Binding Specificity: C-Term

Specificity: MARCH1 Antibody detects endogenous levels of total MARCH1.

Predicted Reactivity: Rabbit,Chicken

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human MARCH1, corresponding to a region within C-terminal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:1000-3000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for MARCH1

Target: MARCH1 (Membrane-Associated Ring Finger (C3HC4) 1, E3 Ubiquitin Protein Ligase (MARCH1))

Alternative Name: MARCH1

Background:

Description: E3 ubiquitin-protein ligase that mediates ubiquitination of TFRC, CD86, FAS and MHC class II proteins, such as HLA-DR alpha and beta, and promotes their subsequent endocytosis and sorting to lysosomes via multivesicular bodies. By constitutively ubiquitinating MHC class II proteins in immature dendritic cells, down-regulates their cell surface localization thus sequestering them in the intracellular endosomal system.

Gene: 1-Mar

Molecular Weight: 32 kDa

Gene ID: 55016

UniProt: Q8TCQ1