MAP/microtubule Affinity-Regulating Kinase 1 (MARK1) antibody

Antigen: MAP/microtubule Affinity-Regulating Kinase 1 (MARK1)

Synonyms: MARK, KIAA1477, MGC126512, MGC126513, Emk3, AW491150, mKIAA1477, B930025N23Rik, MGC80341, MARK1

Clonality: Polyclonal

Host: Goat

Application: ELISA

Catalog no.: ABIN238668

Additional Information

Alternative name: Mark1 (mouse)

Gene ID: 226778 (mouse), 117016 (rat)

Immunogen: C-EQKEEWDKDTARR

Format: Affinity-purified

Description: All names and symbols: Mark1, MAP/microtubule affinity-regulating kinase 1, AW491150, B930025N23Rik, Emk3, KIAA1477, mKIAA1477. Official Symbol: MARK1. RefSeq number(s): NP_663490.1

Specificity: Mouse, Rat

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:1,000. Western Blot: Preliminary experiments in Brain and Testis lysates from Mouse and Rat gave no specific signal but low background (at antibody concentration up to 1µg/ml). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates?

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Publications

Publications: Marx, Nugoor, Mueller et al.: "Structural variations in the catalytic and ubiquitin-associated domains of microtubule-associated protein/microtubule affinity regulating kinase (MARK) 1 and MARK2." in: The Journal of biological chemistry, Vol. 281, Issue 37, pp. 27586-99, 2006 (PubMed).