Apolipoprotein B MRNA Editing Enzyme, Catalytic Polypeptide-Like 2 (APOBEC2) (N-Term), (Internal Region) antibody

Details for Product No. ABIN238679
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Antigen
Synonyms ARP1, ARCD1, Arp1, MGC84761, arp1, arcd1, MGC89256, APOBEC2, DKFZp468H1727
Epitope
N-Term, Internal Region
(6), (5), (3), (2), (1), (1), (1), (1)
Reactivity
Human
(32), (20), (18), (3), (1)
Host
Goat
(25), (9), (3)
Clonality
Polyclonal
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB)
(26), (12), (10), (9), (4), (3), (2)
Pubmed 1 reference available
Catalog no. ABIN238679
Quantity 100 µg
Price
374.27 $   Plus shipping costs $45.00
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Immunogen Peptide with sequence C-EAASQNGEDLENLDD, from the internal region (near the N Terminus) of the protein sequence according to NP_006780.1.
Cross-Reactivity Mouse (Murine), Rat (Rattus)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name ApoBEC2
Background Synonyms: APOBEC2, apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 2, ARCD1, ARP1, OTTHUMP00000039773, apolipoprotein B mRNA editing enzyme, catalytic polypeptide 2
Gene ID 10930, 11811, 301226
NCBI Accession NP_006780.1
Application Notes ELISA: antibody detection limit dilution 1:1000.
Western blot: Approx 26 kDa band observed in Human Heart and Skeletal Muscle lysates (calculated MW of 25.7 kDa according to NP_006780.1). Recommended concentration: 1-3 µg/mL. An additional band of 90 kDa was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal. We call for caution when used for other assays than Western blot.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
anti-Apolipoprotein B MRNA Editing Enzyme, Catalytic Polypeptide-Like 2 (APOBEC2) (N-Term), (Internal Region) antibody ABIN238679 (1µg/ml) staining of Human Heart lysate (35µg protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.
General Prochnow, Bransteitter, Klein et al.: "The APOBEC-2 crystal structure and functional implications for the deaminase AID." in: Nature, Vol. 445, Issue 7126, pp. 447-51, 2007 (PubMed).

Hosts (25), (9), (3)
Reactivities (32), (20), (18), (3), (1)
Applications (26), (12), (10), (9), (4), (3), (2)
Conjugates (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (6), (5), (3), (2), (1), (1), (1), (1)
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