Peptidylglycine alpha-Amidating Monooxygenase (PAM) antibody

Antigen: Peptidylglycine alpha-Amidating Monooxygenase (PAM)

Synonyms: PAL, PHM, MGC137729, pal, phm, pamb

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN238682

Additional Information

Alternative name: PAM / PAL

Gene ID: 5066

Immunogen: C-DQEKEDDGSESEE

Format: Affinity-purified

Description: All names and symbols: PAM, PAL, peptidylglycine alpha-amidating monooxygenase , PHM, pancreatic peptidylglycine alpha-amidating monooxygenase, peptidyl alpha-amidating enzyme, peptidyl-alpha-hydroxyglycine alpha-amidating lyase, peptidylglycine 2-hydroxylase, peptidylglycine alpha-hydroxylating monooxygenase. Official Symbol: PAM. RefSeq number(s): NP_000910.2, NP_620121.1, NP_620176.1, NP_620177.1

Specificity: This antibody is expected to recognise all reported isoforms (NP_000910.2, NP_620121.1, NP_620176.1 and NP_620177.1). Human

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:4,000. Western Blot: Approx 100kDa band observed in Human Kidney lysates (calculated MW of 101kDa according to preproprotein NP_620121.1). Recommended concentration: 0.3-1µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN238682 (1µg/ml) staining of Human Kidney lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Glauder, Ragg, Rauch et al.: "Human peptidylglycine alpha-amidating monooxygenase: cDNA, cloning and functional expression of a truncated form in COS cells." in: Biochemical and biophysical research communications, Vol. 169, Issue 2, pp. 551-8, 1990 (PubMed).