APRT antibody

Catalog No. ABIN2460045

Product Details anti-APRT Antibody

Target: APRT (Adenine Phosphoribosyltransferase (APRT))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This APRT antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purification: Antibody is purified by peptide affinity chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human APRT.

Application Details

Application Notes: APRT antibody can be used for detection of APRT by ELISA at 1:312500. APRT antibody can be used for detection of APRT by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store APRT antibody at -20 °C.

Target Details for APRT

Target: APRT (Adenine Phosphoribosyltransferase (APRT))

Alternative Name: APRT (APRT Products)

Synonyms: Tb07.43M14.200 antibody, Tb07.43M14.180 antibody, C85684 antibody, AMP antibody, APRTD antibody, adenine phosphoribosyltransferase antibody, adenine phosphoribosyl transferase antibody, CND05020 antibody, Tb927.7.1790 antibody, Tb927.7.1780 antibody, Arnit_0941 antibody, Saut_1231 antibody, Fbal_1180 antibody, PH_RS07970 antibody, PF_RS08760 antibody, PAB_RS02560 antibody, Aprt antibody, APRT antibody

Background: Adenine phosphoribosyltransferase (APRT) belongs to the purine/pyrimidine phosphoribosyltransferase family. A conserved feature of this gene is the distribution of CpG dinucleotides. This enzyme catalyzes the formation of AMP and inorganic pyrophosphate from adenine and 5-phosphoribosyl-1-pyrophosphate (PRPP). It also produces adenine as a by-product of the polyamine biosynthesis pathway. A homozygous deficiency in this enzyme causes 2,8-dihydroxyadenine urolithiasis. Two transcript variants encoding different isoforms have been found for this gene.Adenine phosphoribosyltransferase belongs to the purine/pyrimidine phosphoribosyltransferase family. A conserved feature of this gene is the distribution of CpG dinucleotides. This enzyme catalyzes the formation of AMP and inorganic pyrophosphate from adenine and 5-phosphoribosyl-1-pyrophosphate (PRPP). It also produces adenine as a by-product of the polyamine biosynthesis pathway. A homozygous deficiency in this enzyme causes 2,8-dihydroxyadenine urolithiasis. Two transcript variants encoding different isoforms have been found for this gene.

Molecular Weight: 20 kDa

Gene ID: 353

NCBI Accession: NP_000476

UniProt: P07741

Pathways: Ribonucleoside Biosynthetic Process