Fusion protein containing 120aa amino acids from N Terminal region (according to NP_003738.2)
Purification
TNKS antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
TNKS antibody was raised against an 11 amino acid synthetic peptide near the N Terminal Region of TNKS.
Western Blot: In transfected HEK293 transiently expressing TANK1 a band of approximately 142 kDa is observed. This band is not observed in the non-transfected HEK293. The calculated molecular size is 142 kDa according to NP_003738.2. Recommended concentration, 0.5-1.5 μg/mL. Full image caption: HEK293 overexpressing TANK1 (lane 2) and TANK2 (lane 4) and probed with 42-019 (mock transfection in first lane). Lane three is empty. Lower panel shows the same lysates probed for alpha-Actin to show protein levels. Primary incubation (0.5 μg/mL) was overnight at 4 °C. Detected by chemiluminescence.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.