CPT2 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
CPT2 antibody was raised against a 14 amino acid synthetic peptide near the internal region of CPT2 (aa 141-154).
CPT2
Reactivity: Human
WB
Host: Rabbit
Polyclonal
unconjugated
Application Notes
ELISA: Antibody detection limit dilution 1:8000 Western Blot: Approximately 70 kDa band observed in human and mouse liver lysates (calculated MW of 73.8 kDa according to human NP_000089.1 and of 74.0 kDa according to mouse NP_034079.2). Recommended concentration: 1-3 μg/mL. an additional band of unknown identity was also consistently observed at 26 kDa . This band was successfully blocked by incubation with the immunizing peptide.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.