Pyruvate Dehydrogenase (C-Term) antibody
Reactivity: Human
WB, IHC, ELISA
Host: Goat
Polyclonal
unconjugated
-
- Target
- Pyruvate Dehydrogenase
- Binding Specificity
- C-Term
-
Reactivity
- Human
-
Host
- Goat
-
Clonality
- Polyclonal
-
Conjugate
- Un-conjugated
-
Application
- Western Blotting (WB), Immunohistochemistry (IHC), ELISA
- Specificity
- This antibody is expected to recognise all reported isoforms.
- Purification
- Pyruvate Dehydrogenase antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Immunogen
- Pyruvate Dehydrogenase antibody was raised against a 14 amino acid synthetic peptide near the C-Terminus of Pyruvate Dehydrogenase.
-
-
- Application Notes
- ELISA: Antibody detection limit dilution 1:32000. Western Blot: Approximately 50 kDa band observed in human pancreas lysate (calculated MW of 54.2 kDa according to NP_003468.1). In transfected HEK293 transiently expressing PDHX a band of approximately 50 kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.1-1 μg/mL. Immunohistochemistry: In paraffin embedded human pancreas, shows cytoplasmic staining of acinar cells, islets of Langerhans and peri-pancreatic fat cells. Recommended concentration, 1-3 μg/mL.
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- 500 μg/mL
- Buffer
- Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Minimize freezing and thawing.
- Storage
- -20 °C
- Storage Comment
- Aliquot and store at -20 °C.
-
-
You are here:
