ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
Purification
ABTB1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
ABTB1 antibody was raised against a 14 amino acid synthetic peptide near the C-Terminus of ABTB1.
ABTB1
Reactivity: Human
ELISA, WB, IF (cc), IF (p), IHC (p), IHC (fro), ICC
Host: Rabbit
Polyclonal
unconjugated
Application Notes
ELISA: Antibody detection limit dilution 1:32,000. Western Blot: Approximately 40 kDa band observed in human heart lysates (predicted MW of 39 kDa according to NP_115937). Recommended for use at 1-3 μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Minimize freezing and thawing.
Storage
-20 °C
Storage Comment
Aliquot and store at -20 °C.
Target
ABTB1
(Ankyrin Repeat and BTB (POZ) Domain Containing 1 (ABTB1))