Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
Specificity
This epitope was selected to minimise the chance of cross-reactivity with ACTR1A (YEEDGARSIHRKT).
Purification
ARP1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
ARP1 antibody was raised against a 13 amino acid synthetic peptide near the C-Terminus of ARP1.
ANGPTL1
Reactivity: Human
ELISA
Host: Rabbit
Polyclonal
HRP
Application Notes
ELISA: Antibody detection limit dilution 1:128000. Western Blot: Approximately 45 kDa band observed in human heart and brain lysates (predicted MW of 43 kDa according to NP_005726). Recommended for use at 0.5-2 μg/mL. Immunohistochemistry: In paraffin embedded human uterus shows fibrillar staining of cytoplasm in endometrial cells. Recommended concentration, 3-5 μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.