BAZ2B antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
BAZ2B antibody was raised against a 13 amino acid synthetic peptide near the C-Terminus of BAZ2B.
ELISA: Antibody detection limit dilution 1:4,000. Western Blot: Approximately 35 kDa band in testis and brain lysates after 0.3 μg/mL antibody staining. The 35 kDa band was successfully blocked by incubation with the immunizing peptide. Immunohistochemistry: In paraffin embedded human small intestine shows primarily nuclear staining in a number of cells of the lamina propria. Recommended concentration, 1-2 μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Minimize freezing and thawing.
Storage
-20 °C
Storage Comment
Aliquot and store at -20 °C.
Target
BAZ2B
(Bromodomain Adjacent To Zinc Finger Domain, 2B (BAZ2B))