All reported variants represent isoform 1 (NP_001196.1, NP_053581.1, NP_053582.1, NP_053583.1)
Purification
BNIP1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
BNIP1 antibody was raised against a 15 amino acid synthetic peptide near the internal region of BNIP1.
BNIP1
Reactivity: Human
WB
Host: Rabbit
Polyclonal
RB40208
unconjugated
Application Notes
ELISA: Antibody detection limit dilution 1:8,000. Western Blot: Approximately 28 kDa band observed in human kidney lysates (calculated MW of 26.1 kDa according to NP_001196.1, NP_053581.1, NP_053582.1, NP_053583.1). Recommended concentration: 0.5-1.5 μg/mL. an additional band of unknown identity was also consistently observed at 48 kDa . This band was successfully blocked by incubation with the immunizing peptide.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Minimize freezing and thawing.
Storage
-20 °C
Storage Comment
Aliquot and store at -20 °C.
Target
BNIP1
(BCL2/adenovirus E1B 19kDa Interacting Protein 1 (BNIP1))