This antibody is expected to recognize both reported isoforms (NP_005704.1 and NP_112729.1)
Purification
COL4A3BP antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
COL4A3BP antibody was raised against an 11 amino acid synthetic peptide near the internal region of COL4A3BP.
COL4A3BP
Reactivity: Human
WB
Host: Rabbit
Polyclonal
unconjugated
Application Notes
ELISA: Antibody detection limit dilution 1:32,000. Western Blot: Approximately 60 kDa band observed in mouse heart lysates (calculated MW of 71.1 kDa according to mouse NP_075909.1 and 60.0 kDa according to human NP_112729.1). Recommended concentration: 0.5-1.5 μg/mL. an additional band of unknown identity was also consistently observed at 26 kDa . This band was successfully blocked by incubation with the immunizing peptide.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Minimize freezing and thawing.
Storage
-20 °C
Storage Comment
Aliquot and store at -20 °C.
Target
COL4A3BP
(Collagen, Type IV, alpha 3 (Goodpasture Antigen) Binding Protein (COL4A3BP))