Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)
Purification
TRPV5 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
TRPV5 antibody was raised against a 12 amino acid synthetic peptide near the internal region of TRPV5.
TRPV5
Reactivity: Human
WB, ELISA
Host: Mouse
Polyclonal
unconjugated
Application Notes
ELISA: Antibody detection limit dilution 1:16,000. Western Blot: Approximately 85 kDa band observed in human pancreas lysates (calculated MW of 82.6 kDa according to NP_062815.2). Recommended concentration: 1-3 μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.