Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA
Specificity
Note there is a hypothetical protein called similar to vacuolar protein sorting 35 (XP_040192.1), which is virtually identical.
Purification
VPS35 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
VPS35 antibody was raised against a 14 amino acid synthetic peptide near the C-Terminus of VPS35.
ELISA: Antibody detection limit dilution 1:16000. Western Blot: Approximately 90 kDa band observed in human brain and testis lysates (predicted MW of 91 kDa according to NP_060676.2). Recommended for use at 1-3 μg/mL. Immunohistochemistry: In paraffin embedded human testis, shows positive staining in Leydig cells, and cytoplasmic staining (dot-like) of some epithelial cells of the epididymis and of the rete testis.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.